Scientific Journal

Determining the Genetic Diversity of Some Iranian Alyssum spp. Species Using Internal Transcribed Spacer (ITS) Sequences

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Science, Lorestan University, Khorramabad, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract
The genus Alyssum L., a member of the Brassicaceae family, comprises approximately 170 to 195 species worldwide, including 35 taxa native to Iran, 28 recognized as distinct species and 7 varieties. Alyssum is notable for its ecological diversity and pharmacological potential, serving as a valuable reservoir of biodiversity and traditional medicinal applications. Several species have been historically used in traditional medicine, particularly for the treatment of rabies, coughs, and as sedatives. Despite growing scientific interest, the phylogenetic relationships within the genus remain inadequately resolved, largely due to morphological homoplasy and polyphyly observed in key sections such as Odontarrhena and Meniocus. To overcome these challenges, molecular markers have become essential tools in systematic studies, using nuclear ribosomal internal transcribed spacer (ITS) region (ITS1–5.8S–ITS2) proving especially useful information in resolving complex taxonomic relationships. This study represents a pioneering effort to conduct a comprehensive phylogenetic analysis of Iranian Alyssum species using ITS sequence data, with the aim of clarifying taxonomic ambiguities and illuminating their evolutionary relationships.

Keywords


Al-Shehbaz, I.A. (2002). New species of Alyssum, Aphragmus, Arabis, and Sinosophiopsis (Brassicaceae) from China and India. Novon, A Journal for Botanical Nomenclature, 12(3): 309-313. https://doi.org/10.2307/3393071
AL-Shehbaz, I.A. and Ihsan A.A. (2012). Generic and tribal synopsis of the Brassicaceae (Cruciferae). Taxon, 61(5): 931-954. https://doi.org/10.1002/tax.615002
AL-Shehbaz, I.A., Beilstein, M.A. and Kellogg, E.A. (2006). Systematics and phylogeny of the Brassicaceae: an overview. Plant Systematics and Evolution, 259: 89-120. https://doi.org/10.1007/s00606-006-0415-z
Bailey, C.D., Koch, M.A., Mayer, M., Mummenhoff, K., O'kane, S.L.,Warwick, S.I., Windham, M.D. and Al- Shehbaz, I.A. (2006). Toward a global phylogeny of the Brassicaceae. Molecular Biology and Evolution, 23: 2145- 2160. https://doi.org/10.1093/molbev/msl087
Cecchi, L., Gabbrielli, R., Arnetoli, M., Gonnelli, C., Hasko, A. and Selvi, F. (2010). Evolutionary lineages of nickel hyperaccumulation and systematics in European Alysseae (Brassicaceae): evidence from nrDNA sequence data. Annals of Botany, 106(5): 751-67. https://doi.org/10.1093/aob/mcq162
Darvishian, A., Ismaili, A., Nazarian-Firouzabadi, F., MirDrikvand, R. and Hosseinpour, T. (2016). Assessment of genetic diversity among wheat genotypes of west Iran, using randomized markers. Plant Genetic Researches, 2(2): 47-56 (In Persian). https://doi.org/10.29252/pgr.2.2.47
Dudley, T. (1964). Studies in Alyssum: near Eastern representatives and their allies, I. Journal of the Arnold Arboretum, 45: 57-100. https://doi.org/10.5962/p.185679
Eslami-Farouji, A., Khodayari, H., Assadi, M., Çetin, O., Mummenhoff, K. and Özüdoğru, B. (2021). Phylogeny and biogeography of the genus Hesperis (Brassicaceae, tribe Hesperideae) inferred from nuclear ribosomal DNA sequence data. Plant Systematics and Evolution, 307(17): 1-22. https://doi.org/10.1007/s00606-020-01727-y
Franzke, A., Lysak, M.A., Al-Shehbaz, I.A., Koch, M.A. and Mummenhoff, K. (2011). Cabbage family affairs: the evolutionary history of Brassicaceae. Trends in Plant Science, 16: 108-116. https://doi.org/10.1016/j.tplants.2010.11.005
Gawel, N.J., and Jarret, R.L. (1991). A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Molecular Biology Reporter, 9: 262-266. https://doi.org/10.1007/BF02672076
Ghorbani Marghashi, M., Bagheri, H. and Gholami, M. (2019). Identification of some Iranian Cardamine species using the ITS molecular marker, Journal of Plant Research, 32(1): 28-38 (In Persian).
Hendriks, K.P., Kiefer, C., Al-Shehbaz, I.A., Bailey, C.D., Hooft van Huysduynen, A., Nikolov, L.A., Nauheimer, L., Zuntini, A.R., German, D.A., Franzke, A., Koch, M.A., Lysak, M.A., Toro-Núñez, Ó., Özüdoğru, B., Invernón, V.R., Walden, N., Maurin, O., Hay, N.M., Shushkov, P., Mandáková, T., Schranz, M.E., Thulin, M., Windham, M.D., Rešetnik, I., Španiel, S., Ly, E., Pires, J.C., Harkess, A., Neuffer, B., Vogt, R., Bräuchler, C., Rainer, H., Janssens, S.B., Schmull, M., Forrest, A., Guggisberg, A., Zmarzty, S., Lepschi, B.J., Scarlett, N., Stauffer, F.W., Schönberger, I., Heenan, P., Baker, W.J., Forest, F., Mummenhoff, K. and Lens, F. (2023). Global Brassicaceae phylogeny based on filtering of 1,000-gene dataset. Current Biology, 33: 4052-4068.e6. https://doi.org/10.1016/j.cub.2023.08.026
Jomeh Ghasem Abadi, Z., Fakheri, B. and Fazeli-Nasab B. (2019). Study of the molecular diversity of internal transcribed spacer region (ITS1.4) in some lettuce genotypes. Journal of Crop Breeding, 11(29): 29-39 (In Persian). https://doi.org/10.29252/jcb.11.29.29
Kavousi, K., Nazary, Z. and Ghahremani Nejad, F. (2014) New Species of Alyssum (Brassicaceae) from Northeastern Iran. Novon: A Journal for Botanical Nomenclature, 23(1): 59-61. https://doi.org/10.3417/2010116
Koch, M. Kiefer, C. (2006). Molecules and migration: biogeographical studies in cruciferous plants, Plant Systematics and Evolution, 259: 121-142. https://doi.org/10.1007/s00606-006-0416-y
Li, Y., Feng, Y., Lv, G., Liu, B. and Qi, A. (2015). The phylogeny of Alyssum (Brassicaceae) inferred from molecular data. Nordic Journal of Botany, 33: 715-721. https://doi.org/10.1111/njb.00588
Li, Y., Kong, Y., Zhang, Z., Yin, Y., Liu, B., Guanghui, L.V. and Wang, X. (2014). Phylogeny and biogeography of Alyssum (Brassicaceae) based on nuclear ribosomal ITS DNA sequences. Journal of Genetics, 93(2): 313-323. https://doi.org/10.1007/s12041-014-0362-3
Mengoni, A., Baker, A.J.M., Bazzicalupo, M., Reeves, R.D., Adiguzel, N., Chianni, E. et al. (2003). Evolutionary dynamics of nickel hyperaccumulation in Alyssum revealed by ITS nrDNA analysis. New Phytologist, 159: 691-699. https://doi.org/10.1046/j.1469-8137.2003.00837.x
MirMohammadi Maibody, S.A.M. and Golkar, P. (2019) Application of DNA molecular markers in plant breeding. Plant Genetic Researches, 6(1): 1-30 (In Persian). https://doi.org/10.29252/pgr.6.1.1
Mirzadeh Vaghefi, S.S., Asadi, M. and Sheidai, M. (2016). A new species of the genus Alyssum section Alyssum (Brassicaceae) from Iran. Nova Biologica Reperta, 3(2): 145-150. https://doi.org/10.21859/acadpub.nbr.3.2.145
Ozay, C. and Mammadov, R. (2016). Assessment of some biological activities of Alyssum L. known as madwort. Acta Poloniae Pharmaceutica, 73(5): 1213-1220.
Rechinger, K. (1968) Alyssum in Flora Iranica. Cruciferae, 57: 146-170.
Rešetnik, I., Satovic, Z., Schneeweiss, G. M., and Liber, Z. (2013). Phylogenetic relationships in Brassicaceae tribe Alysseae inferred from nuclear ribosomal and chloroplast DNA sequence data. Molecular Phylogenetics and Evolution, 69(3): 772-786. https://doi.org/10.1016/j.ympev.2013.06.026
Španiel, S., Kempa, M., Salmerón-Sánchez, E., Fuertes-Aguilar, J., Mota, J.F., Al-Shehbaz, I.A., German, D.A., Olšavská, K., Šingliarová, B., Zozomová-Lihová, J. and Marhold, K. (2015). AlyBase: database of names, chromosome numbers, and ploidy levels of Alysseae (Brassicaceae), with a new generic concept of the tribe. Plant Systematics and Evolution, 301: 2463-2491. https://doi.org/10.1007/s00606-015-1257-3
Warwick, S., Francis, A. and Al-Shehbaz, I. (2006). Brassicaceae: species checklist and database on CD-Rom. Plant Systematics and Evolution, 259(2-4): 249-258. https://doi.org/10.1007/s00606-006-0422-0
Warwick, S.I., Al-Shehbaz, I.A., Price, R.A. and Sauder, C. (2002). Phylogeny of Sisymbrium (Brassicaceae) based on ITS sequences of nuclear ribosomal DNA. Canadian Journal of Botany, 80: 1002-1017. https://doi.org/10.1139/b02-089
Warwick, S.I., Sauder, C.A. and Al-Shehbaz, I.A. (2008). Phylogenetic relationships in the tribe Alysseae (Brassicaceae) based on nuclear ribosomal ITS DNA sequences. Botany, 86(4): 315-336. https://doi.org/10.1139/B08-013