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:: Volume 5, Issue 1 (2018) ::
pgr 2018, 5(1): 63-76 Back to browse issues page
SNP Marker Assisted Selection for Identification of Fusarium Resistant Melon Plants
Shima Taghikhani , Hossein Ramshini * , Seyed Ahmad Sadat-Noori , Mahmoud Lotfi , Ali Izadi Darbakdi , Naeimeh Sousaraei , Abdollah Varvani Farahani
Department of Agronomy and Plant Breeding Sciences, Aburaihan Campus, University of Tehran, Pakdasht, Iran , ramshini_h@ut.ac.ir
Abstract:   (12444 Views)
Melon is an important crop cultivated in moderate climate regions of the world. One of the most important diseases of this plant is vascular wilt caused by Fusarium oxysporum f.sp. melonis (Fom). Infection of farm by this pathogen can result in huge damage around the world. Development of resistant varieties is the most effective method for disease control. Four races of 0, 1, 2 and 1,2 have been reported for this pathogen and 1 is more versatile and dangerous races especially in the north half of Iran. Garmak, as an important variety in Isfahan province, is susceptible to race 1. Single dominant gene of Fom-2 induces resistance against race 0 and 1. In this research, the resistance gene was transferred from Isabelle into Garmak using SNP marker assisted back-crossing. The screening of plants in BC1, BC2, and BC3 generations was carried out using artificial inoculation. This gene was sequenced partially in parental genotypes and resistant and susceptible alleles were discriminated. In order to verify the resistant plants survived after artificial inoculation in BC3, plants were genotyped using the SNP marker and HRM method. This method distinctly separated three genotypes (RR, Rr and rr) from each other.  The resistance of most plants was verified by molecular method. However, among resistant genotypes identified by artificial inoculation method, there were some susceptible plants identified by molecular method, indicating the more confidence of molecular method in genotyping of plants. With selfing of BC3 resistant plants and selection of homozygote resistant plants, it is possible to develop resistant Garmak variety.
Keywords: Vascular Wilt, Marker Assisted Back-Crossing, Fom-2
Full-Text [PDF 1014 kb]   (2025 Downloads)    
Type of Study: Research | Subject: Plant genetics
References
1. Banihashemi, Z. (2010). Reaction of Cucumis melo cultivars to races of Fusarium oxysporum f. sp. melonis the cause of melon vascular wilt. Iranian Journal of Plant Pathology, 46: 5-7 (In Persian).
2. FAO. (2013). FAOSTAT. Food and Agricultural Organization of the United Nations. Available at http://www.fao.org/faostat/en/#rankings/countries_by_commodity. FAO. Rome, Italy.
3. Foolad, M.R. and Panthee, D.R. (2012). Marker-Assisted selection in tomato breeding (Review). Critical Reviews in Plant Sciences, 31: 93-123. [DOI:10.1080/07352689.2011.616057]
4. Hayashi, K., Yoshida, H. and Ashikawa, I. (2006) Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes. Theoretical and Applied Genetics, 113: 251-260. [DOI:10.1007/s00122-006-0290-6]
5. Han, Y., Khu, D.M. and Monteros, M. (2012). High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.). Molecular Breeding, 29: 489-501. [DOI:10.1007/s11032-011-9566-x]
6. Hwang, J.H., Ahn, S.G., Oh, J.Y., Choi, Y.W., Kang, J.S. and Park, Y.H. (2011). Functional characterization of watermelon (Citrullus lanatus L.) EST-SSR by gel electrophoresis and high resolution melting analysis. Scientia Horticulturea., 130: 715-724. [DOI:10.1016/j.scienta.2011.08.014]
7. Jefferies, S.P., King, B.J., Barr, A.R., Warner, P., Logue, S.J. and Langridge, P. (2003). Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley. Plant Breeding, 122: 52-56. [DOI:10.1046/j.1439-0523.2003.00752.x]
8. Joobeur, T., King, J.J., Nolin, S.J., Thomas, C.E. and Dean, R.A. (2004). The fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. Plant Journal, 39: 283-297. [DOI:10.1111/j.1365-313X.2004.02134.x]
9. Kaviani Charati, A., Sabouri, H., Fallahi, H.A. and Jorjani, E. (2016) QTL Mapping of Spike Characteristics in Barley using F3 and F4 Families Derived from Badia × Komino Cross. Journal of Plant Genetic Research, 3(1): 13-28 (In Persian). [DOI:10.29252/pgr.3.1.13]
10. Khu, Y.H.D. and Monteros, M.J. (2012). High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.). Molecular Breeding, 29: 489-501. [DOI:10.1007/s11032-011-9566-x]
11. Li, Y.H., Zhang, C., Gao, Z.S., Smulders, M.J.M., Ma, Z., Liu, Z.X., Nan, H.Y., Chang, R.Z. and Qiu, L.J. (2009). Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Molecular Breeding, 24: 63-76. [DOI:10.1007/s11032-009-9272-0]
12. Liu, J., Zheng, Z., Zhou, X., Feng, C. and Zhuang, Y. (2014) Improving the resistance of eggplant (Solanum melongena) to Verticillium wilt using wild species Solanum innaeanum. Euphytica, 201: 463-469. [DOI:10.1007/s10681-014-1234-x]
13. Lucchi, C.D., Stevanato, P., Hanson, L. and McGrath, M. (2017). Molecular markers for improving control of soil-borne pathogen Fusarium oxysporum in sugar beet. Euphytica , 213(71): 1-14. [DOI:10.1007/s10681-017-1859-7]
14. Luo, W., Huang, M., Guo, T., Xiao, W., Wang, J., Yang, G., Liu, Y., Wang, H., Chen, Z. and Zhuang, C. (2017). Marker‐assisted selection for rice blast resistance genes Pi2 and Pi9 through high‐resolution melting of a gene‐targeted amplicon. Plant Breeding, 136: 67-73. [DOI:10.1111/pbr.12447]
15. Madadkhah, E., Lotfi, M., Nabipour, A., Rahmanpour, S., Banihashemi, Z. and Shoorooei, M. (2012). Enzymatic activities in roots of melon genotypes infected with Fusarium oxysporum f. sp. melonis race 1. Scientia Horticultura, 135: 171-176. [DOI:10.1016/j.scienta.2011.11.020]
16. Mammadov, J., Chen, W. and Mingus, J. (2012). Development of versatile gene-based SNP assays in maize (Zea mays L.). Molecular Breeding, 29:779-790. [DOI:10.1007/s11032-011-9589-3]
17. Mir Drikvand, R. (2016) Investigation of Heritability of Morphological Traits and Genetic Diversity among Rainfed Barley Genotypes Using Molecular and Morphological Markers. Journal of Plant Genetic Researches, 3(2): 69-82 (In Persian). [DOI:10.29252/pgr.3.2.69]
18. Murray, H.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Research, 8: 4321-4325. [DOI:10.1093/nar/8.19.4321]
19. Oumouloud, A., Arnedo-Andres, M.S., Gonzalez-Torres, R. and Alvarez, J.M. (2008). Development of molecular markers linked to the Fom-1 locus for resistance to Fusarium race 2 in melon. Euphytica, 164: 347-356. [DOI:10.1007/s10681-008-9664-y]
20. Oumouloud, A., El-Otmani, M., Chikh-Rouhou, H., Claver, A.G., Torres, R.G., Perl-Treves, R. and lvarez, J.M. (2013). Breeding melon for resistance to Fusarium wilt: recent developments. Euphytica, 192:155-169. [DOI:10.1007/s10681-013-0904-4]
21. Oumouloud, A., Mokhtari, M., Chikh-Rouhou, H., Arnedo-Andres, M.S., Gonzalez-Torres, R. and Alvarez, J.M. (2012). Characterization of the Fusarium wilt resistance Fom-2 gene in melon. Molecular Breeding, 30: 325-334. [DOI:10.1007/s11032-011-9622-6]
22. Prohens, J. and Nuez, F. (2007). Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Springer Science & Business Media, Berlin, DE. [DOI:10.1007/978-0-387-30443-4]
23. Taylor, C.F. (2009). Mutation scanning using high-resolution melting. Biochemical Society Transactions, 37: 433-437. [DOI:10.1042/BST0370433]
24. Tezuka, T., Waki, K., Kuzuya, M., Ishikawa, T., Takatsu, Y. and Miyagi, M. (2011). Development of new DNA markers linked to the Fusarium wilt resistance locus Fom-1 in melon. Plant Breeding. 130: 261-267. [DOI:10.1111/j.1439-0523.2010.01800.x]
25. Tezuka, T., Waki, K., Yashiro, K., Kuzuya, M., Ishikawa, T., Takatsu, Y. and Miyagi, M. (2009). Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f. sp. melonis race 2 in melon. Euphytica, 168:177-188. [DOI:10.1007/s10681-009-9881-z]
26. Wang, S., Yang, J. and Zhang, M. (2011). Developments of functional markers for Fom-2-mediated fusarium wilt resistance based on single nucleotide polymorphism in melon (Cucumis melo L.). Molecular Breeding, 27: 385-393. [DOI:10.1007/s11032-010-9439-8]
27. Wang, Y.H., Thomas, C.E. and Dean, R.A. (2000). Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.). Molecular Breeding, 6: 379-389.
28. Whitaker, V.M., Bradeen, J.M., Debener, T., Biber, A. and Hokanson, S.C. (2010). Rdr3, a novel locus conferring black spot disease resistance in tetraploid rose: genetic analysis, LRR profiling, and SCAR marker development. Theoretical and Applied Genetics, 120: 573-585. [DOI:10.1007/s00122-009-1177-0]
29. Win, K.M., Korinsak, S., Sirithunya, P., Lanceras-Siangliw, J., Jamboonsri, W., Da, T., Patarapuwadol, S. and Toojinda, T. (2013). Marker assisted introgression of multiple genes for bacterial blight resistance into aromatic Myanmar rice MK-75. Field Crops Research, 154, 164-171. [DOI:10.1016/j.fcr.2013.08.002]
30. Wu, S.B., Franks, T.K., Hunt, P., Wirthensohn, M.G., Gibson, J.P. and Sedgley, M. (2010). Discrimination of SNP genotypes associated with complex haplotypes by high resolution melting analysis in almond: Implications for improved marker efficiencies. Molecular. Breeding, 25: 351-357. [DOI:10.1007/s11032-009-9324-5]
31. Yohannes, T., Tesfamichael, A., Kiambi, D., Folkertsma, R., Tom Hash, C., Ngugi, K., Mutitu, E., Abraha, N., Weldetsion, M., Mugoya, C., Masiga, C.W. and De Villiers, S. (2015). Marker-assisted introgression improves Striga resistance in an eritrean farmer-preferred sorghum variety. Field Crops Research, 173: 22-29. [DOI:10.1016/j.fcr.2014.12.008]
32. Zheng, X.Y., Wolff, D.W., Baudracco-Arnas, S. and Pitrat, M. (1999). Development and utility of cleaved amplified polymorphic sequences (CAPS) and restriction fragment length polymorphisms (RFLPs) linked to the Fom-2 fusarium wilt resistance gene in melon (Cucumis melo L.). Theoretical and Applied Genetics, 99: 453-463. [DOI:10.1007/s001220051257]
33. Zhijuan, J., Jianyao, S., Yuxiang, Z., Qian, Q. and Changdeng, Y. (2014) Application of a simplified marker-assisted backcross technique for hybrid breeding in rice. Biologia 69(4): 463-468. [DOI:10.2478/s11756-014-0335-2]
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Taghikhani S, Ramshini H, Sadat-Noori S A, Lotfi M, Izadi Darbakdi A, Sousaraei N et al . SNP Marker Assisted Selection for Identification of Fusarium Resistant Melon Plants. pgr 2018; 5 (1) :63-76
URL: http://pgr.lu.ac.ir/article-1-123-en.html
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Volume 5, Issue 1 (2018) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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