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:: دوره 8، شماره 1 - ( 1400 ) ::
جلد 8 شماره 1 صفحات 150-133 برگشت به فهرست نسخه ها
تعیین تنوع هاپلوتایپی و ارتباط بین صفات مطلوب زراعی و آلل‌های ریزماهواره واقع بر کروموزوم‌های 4B و 7D گندم نان
اسماعیل دستورانی ، خلیل زینلی نژاد* ، مسعود سلطانی نجف آبادی ، محمدهادی پهلوانی ، حسن سلطانلو ، سعید باقری کیا
گروه اصلاح‌نباتات و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ، zaynalinezhad@gau.ac.ir
چکیده:   (10178 مشاهده)
این پژوهش با هدف تعیین گروه‌های هاپلوتایپی و شناسایی آلل‌های اختصاصی برای صفات مطلوب زراعی گندم نان انجام شد. به‌همین منظور 42 ژنوتیپ گندم بومی ایران و 9 رقم تجاری به‏همراه رقم بهاره چینی (ژنوتیپ مرجع) در قالب طرح آگمنت کشت و از نظر 13 صفت فنوتیپی کمی ارزیابی شدند. نتایج آمار توصیفی نشان داد که صفات طول ریشک و روز تا گلدهی به‌ترتیب بالاترین و پایین‌ترین ضریب تغییرات فنوتیپی را داشتند. به‌منظور بررسی تنوع هاپلوتایپی QTL مرتبط با صفات فنوتیپی واقع بر کروموزوم‌های 4B و 7D گندم نان از هشت نشانگر ریزماهواره استفاده شد. نتایج نشان داد که ژنوتیپ‌های مورد بررسی با توجه به مطابقت آللی با ژنوتیپ مرجع در کروموزوم‌های 4B و 7D به‌ترتیب در 13 و شش گروه هاپلوتایپی قرار گرفتند. به‌منظور بررسی وجود ارتباط بین صفات و نشانگرها، تجزیه واریانس در قالب طرح کاملاً تصادفی با تکرار نامساوی برای تک‌تک نشانگرها انجام شد. در مجموع از 13 صفت مورد بررسی، برای هشت صفت ارتباط معنی‌دار آماری مشاهده شد و برای سه صفت به‌طور هم‌زمان یک آلل اختصاصی معرفی گردید. چنانچه اصلاحگر علاقه‌مند به گزینش ژنوتیپ‌هایی باشد که به‌طور هم‌زمان سه حالت مطلوب یعنی گلدهی زودتر، نیمه‌پاکوتاهی و تعداد بیشتر دانه در سنبله را داشته باشند، می‌تواند از آلل اختصاصی شناسایی شده (153جفتباز) نشانگر Xgwm149-4B استفاده کند.
واژه‌های کلیدی: صفات فنوتیپی، گزینش به کمک نشانگر، گندم بومی، نشانگرهای آلل اختصاصی
متن کامل [PDF 721 kb]   (1434 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک مولکولی
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Dasturani E, Zaynali Nezhad K, Soltani Najafabadi M, Pahlevani M, Soltanlo H, Bagherikia S. Determination of haplotype variation and relationship between desirable agronomic traits and microsatellite alleles on chromosomes 4B and 7D in bread wheat. pgr 2021; 8 (1) :133-150
URL: http://pgr.lu.ac.ir/article-1-214-fa.html

دستورانی اسماعیل، زینلی نژاد خلیل، سلطانی نجف آبادی مسعود، پهلوانی محمدهادی، سلطانلو حسن، باقری کیا سعید. تعیین تنوع هاپلوتایپی و ارتباط بین صفات مطلوب زراعی و آلل‌های ریزماهواره واقع بر کروموزوم‌های 4B و 7D گندم نان. پژوهش های ژنتیک گیاهی. 1400; 8 (1) :133-150

URL: http://pgr.lu.ac.ir/article-1-214-fa.html



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دوره 8، شماره 1 - ( 1400 ) برگشت به فهرست نسخه ها
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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