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Expression Analysis of Gluconase and Metallothionein Genes in Two Wheat Cultivars Under Oxidative Stress
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Mohammad Ehsani   , Saeid Navabpour * , Sayedeh Sanaz Ramzanpour , Hassan Soltanloo , Asadollah Ahmadikhah |
| Department of Plant Breeding and Agricultural Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , s.navabpour@gau.ac.ir |
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Abstract: (135 Views) |
Metal stress is a serious issue that limits the cultivation and yield of crop plants worldwide. It adversely affects plants in different ways, including phenotypic, enzymatic, and gene expression changes. Wheat is an important crop for human nutrition, and the growing world population demands a rapid increase in agricultural products. The rapid advancement of modern industry and agriculture has led to heavy metal pollution due to human activities, posing a serious threat to global health. In this study, a factorial experiment was conducted in a randomized complete block design with three replications. The experimental factors included silver nitrate at three levels (0, 1, 2 mM) as an oxidative stress factor and DABCO as an antioxidant at three levels (0, 10, 20 mM) on wheat cultivars Falat and Tajan. Biochemical traits assessed included the cell oxidation level index and chlorophyll content, and the expression of gluconase and metallothionein genes was evaluated using Real time qRT-PCR. Results of present study showed that the amount of chlorophyll a and b was significantly decreased with increasing in silver nitrate concentration. Pre-treatment with DEBCO prior to silver nitrate foliar application resulted in an increase level of chlorophyll a and b, particularly at 1 mM silver nitrate concentration. Under stress conditions, metallothionein and glucanase gene expression were increased at lower stress levels, whereas their expression was decreased with a higher levels of silver nitrate. Both cultivars showed the highest level of gene expression at 1 mM silver nitrate treatment without DEBCO treatment, followed by significant downregulation at 2 mM silver nitrate treatment. Although this study did not specifically examined the effect of the DEBCO compound, it is well-documented that antioxidants generally reduce oxidative stress in plants. Given the positive impact of DEBCO on alleviating the effects of metal stress, its use as a management method in wheat crops is recommended. Further research on other oxidative stress associated genes can enhance our understanding of plant resistance mechanisms. Exploring the optimal conditions for utilizing silver nitrate and DEBCO can may increase crops yield and enhance the quality of agricultural products. |
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| Keywords: Wheat, Metal stress, Glucanase, Metallothionein, Chlorophyll |
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Type of Study: Research |
Subject:
Molecular genetics
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| Send email to the article author |
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