[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 6, Issue 2 (2020) ::
pgr 2020, 6(2): 43-54 Back to browse issues page
Evaluation of Genetic Diversity among Some Common Fig Using RAPD and ISSR Molecular Markers
Batol Gheitarani , Javad Erfani-Moghadam * , Arash Fazeli
Department of Horticultural Sciences, Ilam University, Ilam, Iran , j.erfani@ilam.ac.ir
Abstract:   (15084 Views)
The common fig (Ficus carica L.), one of the most important fruit species, belongs to Moraceae family and is widely distributed in Iran. In this study, genetic variations among some genotypes of common fig collected from six different regions of Ilam province (Iran) were evaluated based on RAPD and ISSR markers. A total of 73 and 29 alleles were produced by 14 RAPD (with their sizes ranging from 350 to 2500 bp) and 5 ISSR (with their sizes ranging from 150 to 1500 bp) primers, respectively. The number of observed alleles for RAPD primers ranged from 1 (OPA-03) to 9 (OPA-09 and UBC-429), with an average of 5.21 alleles per locus. Also, the number of observed alleles for ISSR primers ranged from 3 (UBC-807) to 8 (UBC-810 and UBC-414), with an average of 5.8 alleles per locus. The highest and lowest values of Shannon's information index (I) was observed in the UBC-429 (2.18) and OPA-03 (0.12) primers, respectively. The Jaccard's genetic similarity coefficient ranged from 0.12 to 0.73 among genotypes based on RAPD data, while for ISSR it was recorded from 0.07 to 1. Also, based on RAPD and ISSR data at a similarity coefficient of 0.45, the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis divided the genotypes into six major groups. As a conclusion, there is a high genetic variability among fig genotypes, which is an important consideration for classification, utilization of germplasm resources and breeding programs of fig.
Keywords: Allele, Genetic variations, Similarity coefficient, Genetic resources
Full-Text [PDF 700 kb]   (2068 Downloads)    
Type of Study: Research | Subject: Molecular genetics
References
1. Aalami, A. and Karami, N. (2016). The study of genetic diversity in Iranian rice cultivars using ISSR, IRAP and REMAP markers. Journal of Crop Breeding, 8: 41-51 (In Persian).
2. Ali-Shtayeh, M.S., Jamous, R.M., Zaitoun, S.Y.A., Mallah, O.B. and Mubaslat, A.K. (2014). Genetic diversity of the palestinian fig (Ficus carica L.) collection by pomological traits and RAPD markers. American Journal of Plant Sciences, 5: 1139-1155. [DOI:10.4236/ajps.2014.59127]
3. Baziar, G., Shahsavar, A.R. and Jafari, M. (2013). Evaluation of genetic diversity on fig (Ficus carica caprificus L.) in Fars province using morphological characteristics and RAPD markers. 1thNational Electronic Conference on New Topics in Gardening Science, Jahrom University of Agricultural Engineering Association, Jahrom, Iran (In Persian).
4. Bendhifi, M., Baraket, G., Zourgui, L., Souid, S. and Salhi-Hannachi, A. (2013). Assessment of genetic diversity of Tunisian Barbary fig (Opuntia ficus indica) cultivars by RAPD markers and morphological traits. Scientia Horticulturae, 158: 1-7. [DOI:10.1016/j.scienta.2013.04.015]
5. Bihamta, M.R., Naserian-Khiabani, B. and Zamani, M.J. (2009). Molecular Markers in Plant Genetics and Biotechnology. University of Tehran Press (UTP), Tehran, IR (In Persian).
6. Botstein, D., White, R.L., Skolnick M. and Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32: 314-331.
7. Cheghamirza, K., Koveza, O., Konovalov, F. and Gostimsky, S. (2002). Identification of RAPD markers and their use for molecular mapping in pea (Pisum sativum L.). Cellular and Molecular Biology Letters, 7: 649-656.
8. Costa, F., Marchese, A., Mafrica, R., Di Vaio, C., Ferrara, G., Fretto, S., Quartararo, A., Marra, F.P., Mennone, C., Vitale, F., Reale, S. and Caruso, T. (2015). Genetic diversity of fig (Ficus carica L.) genotypes grown in southern Italy revealed by the use of SSR markers. Acta Horticulturae, 1173: 75-80. [DOI:10.17660/ActaHortic.2017.1173.13]
9. Doolatian, L., Khodayari, H. and Mohammadi, A. (2017). The genetic diversity survey of the Ficus l. genus in Iran using inter simple sequence repeats markers. Plant Genetic Researches, 4(1): 51-62 (In Persian). [DOI:10.29252/pgr.4.1.51]
10. Essid, A., Aljane, F., Ferchichi, A., and Hormaza, J.I. (2015). Analysis of genetic diversity of Tunisian caprifig (Ficus carica L.) accessions using simple sequence repeat (SSR) markers. Hereditas, 152: 1-7. [DOI:10.1186/s41065-015-0002-9]
11. Ganopoulos, I., Kalivas, A., Kavroulakis, N., Xanthopoulou, A., Mastrogianni, A., Koubouris, G. and Madesis, P. (2015). Genetic diversity of Barbary fig (Opuntia ficus-indica) collection in Greece with ISSR molecular markers. Plant Gene, 2: 29-33. [DOI:10.1016/j.plgene.2015.04.001]
12. Ghorbani, T., Cheghamirza, K. and Arji, E. (2013). Evaluation of genetic diversity in pomegranate using morphological traits and molecular markers. Journal of Agricultural Biotechnology, 5: 111-127 (In Persian).
13. Ikegami, H., Nogata, H., Hirashima, K., Awamura, M. and Nakahara, T. (2009). Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers. Genetic Resources and Crop Evolution, 56: 201-209. [DOI:10.1007/s10722-008-9355-5]
14. Jafari, N., Naderi, P. and Ebrahimzadeh, M.A. (2015). Evaluation of phenolic content, total flavonoid and survey of antioxidant activity of leaves of Ficus carica and Pterocarya fraxinifolia trees using spectrophotometry and high performance liquid chromatograph methods. Iranian Journal of Plant Biology, 7: 1-16 (In Persian).
15. Karimi Shahri, M.R., Dehvari, V., Hajian Shahri, M. and Mokhtarian, A. (2011). Genetic diversity of some grapevine cultivars of Khorasan Razavi province based on RAPD and ISSR markers. Journal of Seed and Plant Improvement, 28: 159- 172 (In Persian).
16. Mahdavian, M., Lessani, H., Ebadi, A. and Fatah, R. (2008). Morphological study of genetic variation among Iranian figs (Ficus carica L.) cultivars. Pajouhesh & Sazandegi, 80: 144-158 (In Persian).
17. MirMohammadi Maibody, S.A.M. and Golkar, P. (2019). Application of DNA molecular markers in plant breeding. Plant Genetic Researches, 6(1): 1-30 (In Persian). [DOI:10.29252/pgr.6.1.1]
18. Murray, M.G. and Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8: 4321-4326. [DOI:10.1093/nar/8.19.4321]
19. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, 70: 3321-3323. [DOI:10.1073/pnas.70.12.3321]
20. Rashidi, A. and Noureddini, M. (2011). Hypoglycemic effect of the aromatic water of leaves of Ficus carica in normal and streptozotocin induced diabetic rats. Pharmacologyonline, 1: 372-379.
21. Saleh, B. (2013). Genetic diversity in Ficus sycomorus L. species (Moraceae) using RAPD and IRAP markers. Agriculture, 59: 120-130. [DOI:10.2478/agri-2013-0011]
22. Salhi-Hannachi, A.M.E.L., Chatti, K., Saddoud, O., Mars, M., Rhouma, A., Marrakchi, M. and Trifi, M. (2006). Genetic diversity of different Tunisian fig (Ficus carica L.) collections revealed by RAPD fingerprints. Hereditas, 143: 15-22. [DOI:10.1111/j.2005.0018-0661.01904.x]
23. Teoman, S., Meryem, I.P.E.K., Erturk, U., Tangu, N.A., Durgut, E., Barut, E., Ercisli, S. and Ipek, A. (2017). Assessment of genetic relationship among male and female fig genotypes using simple sequence repeat (SSR) markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45: 172-178. [DOI:10.15835/nbha45110756]
24. Zhang, P., Dreisigacker, S., Buerkert, A., Alkhanjari, S., Melchinger, A.E. and Warburton, M. L. (2006). Genetic diversity and relationships of wheat landraces from Oman investigated with SSR markers. Genetic Resources and Crop Evolution, 53: 1351-1360. [DOI:10.1007/s10722-005-4675-1]
Send email to the article author



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gheitarani B, Erfani-Moghadam J, Fazeli A. Evaluation of Genetic Diversity among Some Common Fig Using RAPD and ISSR Molecular Markers. pgr 2020; 6 (2) :43-54
URL: http://pgr.lu.ac.ir/article-1-146-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 6, Issue 2 (2020) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657