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:: Volume 8, Issue 1 (2021) ::
pgr 2021, 8(1): 151-168 Back to browse issues page
Investigation of Qualitative Traits and Genes Expression Involved in Bakery Quality for Some of the Bread’s Wheat Doubled Haploid Lines
Mohaddaseh Gholami Farahabadi , Gholam Ali Ranjbar * , Ali Dehestani-Kalagar , Nadali Bagheri
Department of Biotechnology and Plant Breeding, Sari Agriculture and Natural Resources University, Sari, Iran , ali.ranjbar@sanru.ac.ir
Abstract:   (7187 Views)
Bread’s quality depends on wheat flours quality and quantity and for the goal to be achieved, the usage of suitable wheat varieties should be considered. Present study focuses on analyzing doubled-haploid lines of wheat’s bread backing quality and the relationship between qualitative traits and glutenins reservoir proteins. In current work, traits related to bread backing quality of 30 doubled-haploid lines of wheat including their parents and two control varieties (Ehsan and Morvarid) were evaluated. SDS-PAGE test was conducted to identify total amount of protein and the relationship between seeds reservoir proteins and qualitative traits, afterward, a test was conducted to evaluate expression of genes involved in bread backing quality. Results showed that there are significant differences on evaluated traits among all wheat’s genotypes. The highest volume of Zeleny sediment were related to DH-143 and DH-159 (34 and 31 ml, respectively), the highest amount of wet gluten were attributed to DH-159 and DH-143 (77.8 and 74.85 gr, respectively), the highest amount of dry gluten were attributed to DH-159 and DH-143 (26.21 and 25.11 gr, respectively), the highest amount of water absorption percentage were attributed to DH-159 and DH-143 (51.59 and 49.74%, respectively), and the highest percentage of protein content were attributed to DH-143 and DH-159 lines (with the amount of 18.03 and 17.72% respectively). Analyzing of bread backing quality traits indicated that DH-143 and DH-159 were better than the other genotypes. SDS-PAGE test results pointed that the highest amount of seed’s protein is attributed to DH-159 and DH-143 (28.23 and 26.63 µ/gr, respectively). Based on gene expression analysis (using real-time PCR), it was indicated that lines DH-143 and DH-159 had a higher level of expressed than the control treatments for HMW-X, HMW-Y and PDIL genes. Therefore, lines DH-143 and DH-159 could be used in breeding program for optimizing bread backing quality.
Keywords: Gene expression, Protein, Doubled-haploid, Zeleny sediment, SDS-PAGE
Full-Text [PDF 777 kb]   (1625 Downloads)    
Type of Study: Research | Subject: Plant improvement
References
1. Akbari, N., Alavi Kia, S., Norozi, M. and Valizadeh, M. (2017). Relationship between HMW-GS bands and bread making quality traits in recombinant inbred lines derived from a cross between Zagros and Norstar wheat varieties. Cereal Research, 7(2): 185-194 (In Persian).
2. Allahverdiyev, T.I., Talai, J.M., Huseynova, I.M. and Aliyev, J.A. (2015). Effect of drought stress on some physiological parameters, yield, yield components of durum (Triticum durum desf.) and bread (Triticum aestivum L.) wheat genotypes. Ekin Journal of Crop Breeding and Genetics, 1(1): 50-62.
3. Arzani, A. (2002). Grain quality of durum wheat germplasm as affected by heat and drought stress at grain filling period. Wheat Information Service, 94: 9-14.
4. Barnard, A.D., Labuschagne, M.T. and Van Niekerk, H.A. (2002). Heritability estimates of bread wheat quality traits in the Western Cape province of South Africa. Euphytica, 127(1): 115-122.
5. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254.
6. Bushuk, W. (1998). Wheat breeding for end product use. Euphytica. 100: 137-145.
7. Cubadda, R.E., Carcea, M., Marconi, E. and Trivisonno, M.C. (2007). Influence of protein content on durum wheat gluten strength determined by the SDS sedimentation test and by other methods. Cereal Foods World, 52(5): 273-277.
8. Demska, K., Filip, E. and Skuza, L. (2018). Expression of genes encoding protein disulfide isomerase (PDI) in cultivars and lines of common wheat with different baking quality of flour. BMC Plant Biology, 18(1): 294.
9. Dencic, S., Mladenov, N. and Kobiljski, B. (2012). Effects of genotype and environment on breadmaking quality in wheat. International Journal of Plant Production, 5(1): 71-82.
10. Derakhshan, F., Golkari, S. and Sadeghzadeh, B. (2017). Evaluation of the allelic diversity of high-molecular-weight glutenin subunits by SDS-PAGE in cultivar and dryland promising wheat genotypes. Crop Science Research in Arid Regions, 1(2): 222-233 (In Persian).
11. D'ovidio, R., Simeone, M., Masci, S. and Porceddu, E. (1997). Molecular characterization of a LMW-GS gene located on chromosome 1B and the development of primers specific for the Glu-B3 complex locus in durum wheat. Theoretical and Applied Genetics, 95(7): 1119-1126.
12. FAO. (2019). FAO Cereal Supply and Demand Brief. Food and Agriculture Organization of the United Nations. http://www.fao.org/worldfoodsituation/csdb/en/. Accessed 5 December 2019
13. Finney, K.F., Yamazaki, W.T., Youngs, V.L. and Rubenthaler, G.L. (1987). Quality of hard, soft, and durum wheats. Wheat and Wheat Improvement, 13: 677-748.
14. Fowler, D.B., Brydon, J., Darroch, B.A., Entz, M.H. and Johnston, A.M. (1990). Environment and genotype influence on grain protein concentration of wheat and rye. Agronomy Journal, 82(4): 655-664.
15. Ghoreishi, S., Izanloo, A., Parsa, S. and Ghaderi, M.Gh. (2014). Associations between high molecular weight glutenin subunits with bread quality traits of some bread wheat cultivars. Journal of Cereal Research, 4(3): 199-209 (In Persian).
16. Gozubenli, H. (2010). Nitrogen dose and plant density effects on popcorn grain yield. African Journal of Biotechnology, 9(25): 3828-3832.
17. ICC Standard Methods. (2008). ICC Standard Methods. ICC Publication. http://old.icc.or.at/standard_methods. Accessed 12 October 2019.
18. Jockovic, B., Mladenov, N., Hristov, N., Aćin, V. and Djalović, I. (2014). Interrelationship of grain filling rate and other traits that affect the yield of wheat (Triticum aestivum L). Romanian Agricultural Research, 31: 81-87.
19. Joudi, M., Ahmadi, A., Mohammadi, V., Abbasi, A. and Mohammadi, H. (2014). Genetic changes in agronomic and phonologic traits of Iranian wheat cultivars grown in different environmental conditions. Euphytica, 196(2): 237-249.
20. Katagiri, M., Masuda, T., Tani, F. and Kitabatake, N. (2011). Expression and development of wheat proteins during maturation of wheat kernel and the rheological properties of dough prepared from the flour of mature and immature wheat. Food Science and Technology Research, 17(2): 111-120.
21. Khodadadi, E., Aharizad, S., Shahbazi, H. and Sabzi, M. (2013). Heritability of some characters related to bread quality of wheat. Journal of Crop Production and Processing, 3(7): 37-46 (In Persian).
22. Khoshroo, S.M.R., Khavarinejad, R., Baghizadeh, A., Fahimi, H. and Mohammadi, Z.N. (2011). Seed storage protein electrophoretic profiles in some Iranian date palm (Phoenix dactylifera L.) cultivars. African Journal of Biotechnology, 10(77): 17793-17804.
23. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259): 680.
24. Liu, H., Wang, K., Xiao, L., Wang, S., Du, L., Cao, X. and Ye, X. (2016). Comprehensive identification and bread-making quality evaluation of common wheat somatic variation line AS208 on glutenin composition. PloS One, 11(1): e0146933.
25. Ma, W., Appels, R., Bekes, F., Larroque, O., Morell, M.K. and Gale, K.R. (2005). Genetic characterisation of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions. Theoretical and Applied Genetics, 111(3): 410-422.
26. Mehr Azar, A., Mohammadi, M., Najafian, G. and Izadi Darbandi, A. (2014). Relationship between high molecular weight glutenin subunits and grain quality traits in bread wheat cultivars. Behnjadi Journal of Seedlings and Seeds, 29(4): 838-823.
27. Meschibahoush, M., Ranjbar, G., Abbasdokht, H. And Najafi Zarrini, H. (2018). Determination of suitable indices for evaluation of drought tolerance in bread wheat double-haploid lines (Triticum aestivum L.). Crop Physiology Journal. 36(9): 83-99.
28. Motamedzadegan, A. and Hadidi, M. (2012). Protein measurement, basics and measurement methods, 1st. Iran's Agriculture Science, Tehran, Iran (In Persian).
29. Najafian, G., Bahraee, S., Baghaee, N., Morteza-Gholi, M. and Babaee-Goli, E. (2008). Bread making quality attributes of Iranian trade cultivars of wheat and their HMW glutenin subunits composition. The 11th International Wheat Genetics Symposium, Sydney University Press, Sydney, Australian.
30. Najafian, G. and Baghaie, N. (2011). Genetic variation in high molecular weight glutenin subunits in parental lines and cultivars of wheat used in breeding programs of cold and temperate agro-climatic zones of Iran. Seed and Plant Improvement Journal, 27(3): 305- 322 (In Persian).
31. Nakamura, H. (2000). The relationship between high-molecular-weight glutenin subunit composition and the quality of japanese hexaploid wheat lines. Journal of Agricultural and Food Chemistry, 48(7): 2648-2652.
32. Omidvar, A., Abasi, H. and GolAbadi, M. (2018). Investigation the effect of high molecular wheat glutenin subunits on some qualitative characteristics of biscuit. Iranian Journal of Nutrition and Food Industry, 3: 76-65 (In Persian).
33. Parand, M., Yamchi, A., Soltanlu, H. and Zeynalinejad, Kh. (2019). Study of morphological traits and genetic diversity of low molecular wight- glutenin subunits in some bread wheat cultivars using SRAP marker. Journal of Plant Breeding, 26: 55-64 (In Persian).
34. Rajabi Hashjin, M., Fotokian, M.H., Aghaee sarrizeh, M. and Mohamadi, M. (2014). Evaluation of allelic variation and assessment of quality of seed storage proteins in durum wheats. Journal of Agricultural Biotechnology, 5(4): 17-36.
35. Rasheed, A., Xia, X., Yan, Y., Appels, R., Mahmood, T. and He, Z. (2014). Wheat seed storage proteins: advances in molecular genetics, diversity and breeding applications. Journal of Cereal Science, 60(1): 11-24.
36. Rozbicki, J., Ceglińska, A., Gozdowski, D., Jakubczak, M., Cacak-Pietrzak, G., Mądry, W. and Drzazga, T. (2015). Influence of the cultivar, environment and management on the grain yield and bread-making quality in winter wheat. Journal of Cereal Science, 61: 126-132.
37. Sanchez-Garcia, M., Álvaro, F., Peremarti, A., Martín-Sánchez, J.A. and Royo, C. (2015). Changes in bread-making quality attributes of bread wheat varieties cultivated in Spain during the 20th century. European Journal of Agronomy, 63: 79-88.
38. Tanaka, H., Toyoda, S. and Tsujimoto, H. (2005). Diversity of low-molecular-weight glutenin subunit genes in Asian common wheat (Triticum aestivum L.). Breeding Science, 55: 349-354.
39. Wang, P., Chen, H., Mohanad, B., Xu, L., Ning, Y., Xu, J. and Xu, X. (2014). Effect of frozen storage on physico-chemistry of wheat gluten proteins. Studies on gluten, glutenin-and gliadin-rich fractions. Food Hydrocolloids, 39: 187-194.
40. Wang, S., Yu, Z., Cao, M., Shen, X., Li, N., Li, X. and Yan, Y. (2013). Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat breadmaking quality. PLoS One, 8(4): 0058947.
41. Yan, Y., Hsam, S.L.K., Yu, J., Jiang, Y. and Zeller, F.J. (2003). Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis. Euphytica, 130(3): 377-385.
42. Zargani, M., Ranjbar, G. and Ebrahim Nezhad, S. (2015). Molecular assessment of genetic diversity among bread wheat (Triticum aestivum L.) doubled haploid lines using SSR markers. Journal of Crop Breeding, 7(15): 88-95 (In Persian).
43. Zhang, L.L., Zhang, Y.B., Song, Q.J., Zhao, H.B., Yu, H.Y., Zhang, C.L. and Xiao, Z.M. (2008). Study on the quality of NILS of wheat cv. Longfumai 3 possessing HMW-GS Null and 1 subunits. Agricultural Sciences in China, 7(2): 140-147.
44. Zhong, Y., Xu, D., Hebelstrup, K.H., Yang, D., Cai, J., Wang, X. and Jiang, D. (2018). Nitrogen topdressing timing modifies free amino acids profiles and storage protein gene expression in wheat grain. BMC Plant Biology, 18(1): 1-14.
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Gholami Farahabadi M, Ranjbar G A, Dehestani-Kalagar A, Bagheri N. Investigation of Qualitative Traits and Genes Expression Involved in Bakery Quality for Some of the Bread’s Wheat Doubled Haploid Lines. pgr 2021; 8 (1) :151-168
URL: http://pgr.lu.ac.ir/article-1-205-en.html


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Volume 8, Issue 1 (2021) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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