Study of Genetic Diversity of Some Allium L. Species Based on ISSR Markers in Kurdistan Province

Hosseini, Shahla; Rahgozar, Mohammad Reza; Badakhshan, Hedieh

doi:10.52547/pgr.8.2.5

[Home ] [Archive]

[ فارسی ]

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Volume 8, Issue 2 (2022)

pgr 2022, 8(2): 57-68

Back to browse issues page

Study of Genetic Diversity of Some Allium L. Species Based on ISSR Markers in Kurdistan Province

Shahla Hosseini ^*

, Mohammad Reza Rahgozar

, Hedieh Badakhshan

Department of Biological Science, Faculty of Basic Sciences, University of Kurdistan, Sanandaj, Iran , sh.hosseini@uok.ac.ir

Abstract: (5406 Views)

Genus Allium L. contains very taxonomically complex sections, especially the subgenus Melanocrommyum. The systematic position of the species in each section has been revised many times over time. In the present study, the relationship between 32 ecotypes belonging to 10 different species of Allium was investigated using ISSR markers. The nine primers used produced 166 polymorphic bands (average 18 bands per primer). Among the primers used, ISSR873 primer with 27 bands made the most, and ISSR4 primer with two bands had the lowest polymorphic bands. The PIC of the markers ranged from 0.04 to 0.43. Cluster analysis by UPGMA method based on molecular markers divided the studied ecotypes into four groups. The clustering and principal coordinate analysis results showed that most morphologically similar species were grouped in closed clusters. According to Dice similarity coefficient, the highest percentage of similarity was shown between Allium stipitatum and Allium saralicum ecotypes (72 percent) from the Melanocrommyum subgenus, and the lowest similarity was obtained between Allium tripedale and Allium iranicum ecotypes (12 percent). The ecotypes with the lowest similarity percentage belong to the subgenus Allium and Nectaroscordum, which are placed in separate clusters. Based on the results, the ecotypes of Pseudoprason, Melanocrommyum, and Procerallium sections showed the highest affinity. In general, it can be concluded that ISSR markers are useful for classifying Allium species and have sufficient potential for phylogenetic studies of species. In addition, due to significant genetic diversity among the studied ecotypes of wild Allium species, this diversity can be used in future breeding programs of crop.

Keywords: Cluster analysis, Genetic diversity, Melanocrommyum

Full-Text [PDF 1202 kb] (1181 Downloads)

Type of Study: Research | Subject: Molecular genetics

References

1. Akhavan, A., Saeidi, H., Rahiminejad, M.R., Zarre, S. and Blattner, F.R. (2015). Interspecific relationships in Allium subgenus Melanocrommyum sections Acanthoprason and Asteroprason (Amaryllidaceae) revealed using ISSR markers. Systematic Botany, 40: 706-715. [DOI:10.1600/036364415X689168]

2. Eghlima, G., Kheiry, A., Sanikhani, M., Hadian, J. and Aelaie, M. (2021). study of genetic diversity of glycyrrizha glabra l. populations using ISSR molecular markers. Plant Genetic Researches, 8: 81-94 (In Persian). [DOI:10.52547/pgr.8.1.6]

3. Friesen, N., Fritsch, R.M. and Blattner, F.R. (2006). Phylogeny and new intrageneric classification of Allium (Alliaceae) based on nuclear ribosomal DNA ITS sequences. Aliso: A Journal of Systematic and Evolutionary Botany, 22: 372-395. [DOI:10.5642/aliso.20062201.31]

4. Fritsch, M.R. and Gurushidze, M. (2009). Phylogenetic relationships of ornamental species in Allium L. subg. Melanocrommyum (Webb et Berthel.) Rouy (Alliaceae). Israel Journal of Plant Sciences, 57: 287-295. [DOI:10.1560/IJPS.57.4.287]

5. Fritsch, R. and Abbasi, M. (2013). A Taxonomic Review of Allium subg. Melanocrommyum in Iran. IPK, Gatersleben, DE.

6. Fritsch, R. and Amini Rad, M. (2013). Allium pseudostrictum (Amaryllidaceae), a new record from Iran. Rostaniha, 14: 81-84.

7. Fritsch, R.M. (2012). Illustrated key to the sections and subsections and brief general circumscription of Allium subg. Melanocrommyum. Phyton (Horn), 52: 1-37.

8. Fritsch, R.M. and Maroofi, H. (2010). New species and new records of Allium L. (Alliaceae) from Iran. Phyton (Horn), 50: 1-26.

9. Gorji, A.M., Poczai, P., Polgar, Z. and Taller, J. (2011). Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. American Journal of Potato Research, 88: 226-237. [DOI:10.1007/s12230-011-9187-2]

10. Gurushidze, M. (2009). Phylogenetic relationships and diversification processes in Allium subgenus Melanocrommyum. Ph.D. Thesis, der Martin-Luther-Universität Halle-Wittenberg, German State of Saxony-Anhalt, Germany.

11. Gurushidze, M., Fritsch, R.M. and Blattner, F.R. (2008). Phylogenetic analysis of Allium subg. Melanocrommyum infers cryptic species and demands a new sectional classification. Molecular Phylogenetics and Evolution, 49: 997-1007. [DOI:10.1016/j.ympev.2008.09.003]

12. Gurushidze, M., Fritsch, R.M. and Blattner, F.R. (2010). Species-level phylogeny of Allium subgenus Melanocrommyum: Incomplete lineage sorting, hybridization and trnF gene duplication. Taxon, 59: 829-840. [DOI:10.1002/tax.593012]

13. Hosseini, S. (2018). Karyological studies of some Allium L.(Amaryllidaceae) species in Iran. The Iranian Journal of Botany, 24: 65-71.

14. Hosseini, S. and Go, R. (2010). Cytogenetic study of some Allium species (subgenus Allium and Melanocrommyum) in Iran. Cytologia, 75: 99-108. [DOI:10.1508/cytologia.75.99]

15. Li, Q.Q., Zhou, S.D., He, X.J., Yu, Y., Zhang, Y C. and Wei, X. Q. (2010). Phylogeny and biogeography of Allium (Amaryllidaceae: Allieae) based on nuclear ribosomal internal transcribed spacer and chloroplast rps16 sequences, focusing on the inclusion of species endemic to China. Annals of Botany, 106: 709-733. [DOI:10.1093/aob/mcq177]

16. Liang, J.X., Qi, J.M., Fang, P.P., Wang, T., Chen, S.H., Zhou, D.X., Tao, A.F., Liang, K.J. and Wu, W.R. (2008). Genetic diversity and genetic relatives analysis of tobacco germplasm based on inter-simple sequence repeat (ISSR). Scientia Agricultura Sinica, 32(3): 373-378.

17. Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.

18. Memariani, F., Joharchi, M.R. and Arjmandi, A.A. (2012). Allium aladaghense (Amaryllidaceae, Allieae), a new species of section Asteroprason from northeast of Iran. Phytotaxa, 56: 28-34. [DOI:10.11646/phytotaxa.56.1.7]

19. Navabpour, S., Yamchi, A. and Golcheshmeh, S. (2021). Assessment of genetic diversity between different accessions of calotropis procera with ISSR molecular markers. Plant Genetic Researches, 8: 17-28 (In Persian). [DOI:10.52547/pgr.8.1.2]

20. Pawell, W., Morganet, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalaski, A. (1996). The comparision of RFLP, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238. [DOI:10.1007/BF00564200]

21. Peakall, R. and Smouse, P.E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6: 288-295. [DOI:10.1111/j.1471-8286.2005.01155.x]

22. Peakall, R. and Smouse, P.E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28: 2537-2539. [DOI:10.1093/bioinformatics/bts460]

23. Petrova, G., Petrova, S., Delcheva, M. and Bancheva, S. (2017). Genetic diversity and conservation of Bulgarian endemic Verbasum tzar-borisii (Scrophulariaceae). Annales Botanici Fennici, 54: 307- 317. [DOI:10.5735/085.054.0614]

24. Razyfard, H., Zarre, S., Fritsch, R.M. and Maroofi, H. (2011). Four new species of Allium (Alliaceae) from Iran. Annales Botanici Fennici, 48: 352-360. [DOI:10.5735/085.048.0407]

25. Ren, N. and Timko, M.P. (2001). AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species. Genome, 44: 559-571. [DOI:10.1139/g01-060]

26. Rezaei, J., Mehrjerdi, Z.M. and Mastali, H. (2018). ISSR based analysis of genetic diversity in some endangered species of Allium subg. Melanocrommyum. Genetika, 50: 59-68. [DOI:10.2298/GENSR1801059R]

27. Rohlf, F.J. (2000). NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, New York, USA.

28. Samiei, L., Kiani, M., Zarghami, H., Memariani, F. and Joharchi, M.R. (2015). Genetic diversity and interspecific relationships of some Allium species using inter simple sequence repeat markers. Bangladesh Journal of Plant Taxonomy, 22: 67-75. [DOI:10.3329/bjpt.v22i2.26029]

29. Santhosh, W., Shobha, D. and Melwyn, G. (2009). Assessment of genetic diversity in cashew germplasm using RAPD and ISSR markers. Scientia Horticulturae, 120: 411-417. [DOI:10.1016/j.scienta.2008.11.022]

30. Shannon, C.E. (1948). A mathematical theory of communication. The Bell System Technical Journal, 27: 379-423. [DOI:10.1002/j.1538-7305.1948.tb01338.x]

31. Sharma, V.R., Malik, S., Kumar, M., Sirohi, A. and Nagaraju, K. (2016). Assessment of genetic diversity in garlic (Allium sativum L.) genotypes based on ISSR markers. Plant Archives, 16: 88-95.

32. Sudha, G.S., Ramesh, P., Sekhar, A.C., Krishna, T.S., Bramhachari, P. and Riazunnisa, K. (2019). Genetic diversity analysis of selected Onion (Allium cepa L.) germplasm using specific RAPD and ISSR polymorphism markers. Biocatalysis and Agricultural Biotechnology, 17: 110-118. [DOI:10.1016/j.bcab.2018.11.007]

33. Zietkiewicz, E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176-183. [DOI:10.1006/geno.1994.1151]

Send email to the article author

‎ 10.52547/pgr.8.2.5

‎ 20.1001.1.23831367.1400.8.2.4.0

Mendeley

Zotero

RefWorks

Hosseini S, Rahgozar M R, Badakhshan H. Study of Genetic Diversity of Some Allium L. Species Based on ISSR Markers in Kurdistan Province. pgr 2022; 8 (2) :57-68
URL: http://pgr.lu.ac.ir/article-1-230-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 8, Issue 2 (2022)

Back to browse issues page

Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657