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:: Volume 5, Issue 1 (2018) ::
pgr 2018, 5(1): 77-86 Back to browse issues page
Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks
Shahin Jahangirzadeh Khiavi * , Zabihollah Zamani , Mohamadreza Fatahi , Masoumeh Ashourpour
Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran , sh.jahangirzadeh@ areeo.ac.ir
Abstract:   (14099 Views)
The chloroplastic DNA of 64 accessions of apple (Malus spp.) (54 Iranian genotypes, five commercial cultivars and five rootstocks) were analyzed to reveal their haplotypes by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Approximately 4320 bp of the chloroplast genome was analyzed, by using two chloroplast universal primer pairs and two restriction enzymes (EcoRI and MseI). All the mutations detected were insertion-deletions been in the range between 10-40 bp. The combination of all the mutations resulted to six haplotypes (H1, H2, H3, H4, H5 and H6) in the studied apple accessions. It was defined that the Iranian apple rootstock 'Gami-Almasi' had a specific haplotype form all the other studied accessions. It was approved that cpDNA diversity data can be considered for phylogenetic studies in this plant and the polymorphism determined in cytoplasmic genome by PCR-RFLP method can help to understand the maternal inheritance in apple.
Keywords: Chloroplast universal primer, Restriction enzyme, Malus Spp, Haplotype, PCR-RFLP
Full-Text [PDF 565 kb]   (1608 Downloads)    
Type of Study: Research | Subject: Plant genetics
References
1. Bagheri, A., Kochaki, A. and Zand, A. (1996). Plant Breeding in Sustainable Agriculture. Mashhad University Jihad publications. Mashhad, IR (In Persian).
2. Bouhadida, M., Martín. J.P., Eremin, G., Pinochet, J., Moreno, M.Á. and Gogorcena, Y. (2007). Chloroplast DNA diversity in Prunus and its implication on genetic relationships. Journal of the American Society for Horticultural Science, 132(5): 670-679. [DOI:10.21273/JASHS.132.5.670]
3. Coart, E., Van Glabeke, S., De loose, M., Larsen, A.S. and Roldan-ruiz, I. (2006). Chloroplast diversity in the genus Malus: New insights into the relationship between the European wild apples (Malus sylvestris (L.) Mill.) and the domesticated apple (Malus domesticaBorkh.). Molecular Ecology, 15: 2171-2182. [DOI:10.1111/j.1365-294X.2006.02924.x]
4. Cros, J., Combes, M.C., Trouslot, P., Anthony, F., Hamon, S., Charrier, A. and Lashermes, P. (1998). Phylogenetic analysis of chloroplast DNA variation in CoffeaL. Molecular Phylogenetics and Evolution, 9(1): 109-117. [DOI:10.1006/mpev.1997.0453]
5. Curtis, S.E. and Clegg, M.T. (1984). Molecular evolution of chloroplast DNA sequences. Molecular Biology and Evolution, 1: 291-301.
6. Demesure, B., Sodzi, N. and Petit, R.J. (1995). A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Molecular Ecology, 4: 129-131. [DOI:10.1111/j.1365-294X.1995.tb00201.x]
7. Dumolin-Lapegue, S., Pemonge, M.H. and Petit, R.J. (1997). An enlarged set of consensus primers for the study of organelle DNA in plants. Molecular Ecology, 6: 393-397. [DOI:10.1046/j.1365-294X.1997.00193.x]
8. Fujii, N., Tomaru, N., Okuyama, K., Koike, T., Mikami, T., and Ueda, K. (2002). Chloroplast DNA phylogeography of Faguscreneta (Fagaceae) in Japan. Plant Systematics Evolution, 232(1-2): 21-33. [DOI:10.1007/s006060200024]
9. Gielly, L. and Taberlet, P. (1994). The use of chloroplast DNA to resolve plant phylogenies. Non-coding vs. Rbcl sequences. Molecular Biology and Evolution, 11: 769-777.
10. Grivet, D., Heinze, B., Vendraminà, G.G. and Petit, R.J. (2001). Genome walking with consensus primers: Application to the large single copy region of chloroplast DNA. Molecular Ecology Notes, 1: 345-349. [DOI:10.1046/j.1471-8278.2001.00107.x]
11. Heuertz, M., Fineschi, S., Anzidei, M., Pastorelli, R., Salvini, D., Paule, L., Frascaria-Lacoste, N., Hardy, O.J., Vekemans, X. and Vendramin, G.G. (2004). Chloroplast DNA variation and postglacial recolonization of common ash (Fraxinus excelsior L.) in Europe. Molecular Ecology, 13: 3437-3452. [DOI:10.1111/j.1365-294X.2004.02333.x]
12. Ishikawa, S., Kato, S., Imakawa, S., Mikami, T. and Shimamoto, Y. (1992). Organel DNA polymorphism in apple cultivars and rootstocks. Theoretical and Applied Genetics, 83: 963-967. [DOI:10.1007/BF00232957]
13. Jahangirzadeh Khiavi, Sh., Zamani, Z., Mardi, M. and Fatahi Moghdam, M. (2013). Evaluation of Chloroplast relationship between some apple genotype from Azerbaijan of Iran and their comparison with other local genotypes, cultivars and rootstocks. African Journal of Agricultural Research, 8(1): 106-112.
14. Katayama, H. and Uematsu, C. (2005). Structural analysis of chloroplast DNA in Prunus (Rosaceae): Evolution, genetic diversity and unequal mutations. Theoretical and Applied Genetics, 111:1430-1439. [DOI:10.1007/s00122-005-0075-3]
15. Kato, S., Ishikawa, S., Imakawa, S., Komori, S., Mikami, T. and Shimamoto, Y. (1993). Mitochondrial DNA restriction fragment length polymorphisms in Malus species. Plant Breeding, 111: 162- 165. [DOI:10.1111/j.1439-0523.1993.tb00623.x]
16. Kucuk, O., Gulbitti-Onarici, S. and Sumer, S. (2006). RFLP of analyses of an intergenic spacer region of chloroplast DNA in some wild wheat species. African Journal of Biotechnology, 5(22): 2058-2061.
17. Manie, A. (2001). Apple and its Culture. Technical Publications Companies in Iran. Tehran, IR (In Persian).
18. Matsumoto, S., Wakita, H. and Soejima, J. (1997). Chloroplast DNA probes as an aid in the molecular classification of Malus species. Scientia Horticulturae, 70: 81-86. [DOI:10.1016/S0304-4238(97)00061-7]
19. Mohanty, A., Martín, J.P. and Aguinagalde, I. (2001). Chloroplast DNA study in wild populations and some cultivars of Prunusavium L. Theoretical and Applied Genetics, 103: 112-117. [DOI:10.1007/s001220000532]
20. Palmer, J.D. (1987). Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. American Society of Naturalists, 130: S6-S29. [DOI:10.1086/284689]
21. Panda, S., Pedro Martin, J. and Aguinaglde, I. (2003). Chloroplast DNA study in sweet cherry cultivars (Prunusavium L.) using PCR-RFLP method. Genetic Resources and Crop Evolution, 50: 489-495. [DOI:10.1023/A:1023986416037]
22. Salehi Shanjani, P. (2004). Chloroplast DNA phylogeography of Fagus orrientalisLipsky in Iran. Journal of Iran Biology, 17(2): 117-126 (In Persian).
23. Taberlet, P., Gielly, L., Pautou, G. and Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17: 1105-1109. [DOI:10.1007/BF00037152]
24. Turkec, A., Sayar, M. and Heinze, B. (2006). Identification of sweet cherry cultivars (Prunusavium L.) and analysis of their genetic relationships by chloroplast sequence-characterised amplified regions (cpSCAR). Genetic Resources and Crop Evolution, 53: 1635-1641. [DOI:10.1007/s10722-005-2285-6]
25. Widmer, A. and Baltisberger, M. (1999). Extensive intraspecific chloroplast DNA (cpDNA) variation in the alpine Drabaaizoides L. (Brassicaceae): Haplotype relationships and population structure. Molecular Ecology, 8: 1405-1415. [DOI:10.1046/j.1365-294x.1999.00700.x]
26. Zeinalabedini, M., Mahjourhat, K., Khayam-Nekoui, M., Grigorian, V., Toorchi, M., Dicenta, F. and Martinez-Gomez, P. (2007). Molecular characterization of almond cultivars and related wild species using nuclear and chloroplast DNA markers. Journal of Food, Agriculture & Environment, 5(3&4): 242-247.
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Jahangirzadeh Khiavi S, Zamani Z, Fatahi M, Ashourpour M. Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks. pgr 2018; 5 (1) :77-86
URL: http://pgr.lu.ac.ir/article-1-124-en.html


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Volume 5, Issue 1 (2018) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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