[Home ] [Archive]   [ فارسی ]  
:: About :: Main :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..



 
..
:: Volume 1, Issue 1 (2014) ::
pgr 2014, 1(1): 13-26 Back to browse issues page
Genetic Diversity Structure of Aegilops cylindrica Accessions Revealed by Genomic ISSR Markers
Samira Mohammadi , Ali Ashraf Mehrabi * , Ali Arminian , Arash Fazeli
Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam , a.mehrabi@mail.ilam.ac.ir
Abstract:   (33491 Views)
Genetic diversity among 35 accessions of Ae. cylindrica using 17 ISSR primers were investigated. Totally, 190 alleles were amplified and 188 alleles (98.95%) o were polymorphic. Number of Amplified alleles ranged from 6 to 20 with average 11.18 alleles for each primer. Polymorphic information content (PIC) varied from 0.10 (primer UBC841) to 0.35 (primer UBC836). Marker index criterion ranged from 0.6 (primer UBC841) to 6 (primer 15). Cluster and Principal Coordinate Analysis could not completely separate accessions and showed no association between molecular diversity and geographic diversity of genotypes, indicating that there is high genetic diversity among accessions. West and south-west genotypes showed more diversity than genotypes from north and north-west of the country. Therefore, the center of diversity and origin of Aegilops cylindrica might be the western and south-western regions of country and this species might transferred from these regions to the northern parts of the country. Results of this study showed that ISSR markers are useful tools for management of genetic resources of wheat and their wild relative species.
Keywords: Aegilops cylindrica, Cluster analysis, Genetic diversity, ISSR marker, Principal coordinate analysis
Full-Text [PDF 1138 kb]   (5058 Downloads)    
Type of Study: Research | Subject: Plant improvement
References
1. Anderson, J.A., Church, G.A., Autrique, J.E., Thanksley, S.D. and Sorrells, M.E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36: 181-86.
2. Barbara, F.R., Brian, L.J. and Jonathan, D.C. (2012). MINITAB Handbook: Update for Release 16. Web site at URL: http://www.minitab.com/en. Accessed 15 Aug 2013.
3. Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
4. El Bouhssini, M., Benlhabib, O., Nachit, M.N., Houari, A., Bentika, A., Nsarellah, N. and Lhaloui, S. (1998). Identification in Aegilops species of resistant sources to Hessian fly (Diptera: Cecidomyiidae) in Morocco. Genetic Resources and Crop Evolution, 45: 343-45.
5. Farkhari, M., Naghavi, M.R., Pyghambari, S.A. and Sabokdast, M. (2007). Genetic variation of Jointed Goatgrass (Aegilops cylindrica Host.) from Iran, using RAPD-PCR and SDS-PAGE of Seed Proteins. Pakistan Journal of Biological Sciences, 10: 2868-73.
6. Farooq, S., Iqbal, N., Asghar, M. and Shah, T.M. (1992). Intergeneric hybridization for wheat improvement VI. Production of salt tolerant germplasm through crossing wheat (Triticum aestivum) with Aegilops cylindrica and its significance in practical agriculture. Journal of Genetics and Plant Breeding, 46: 125-32.
7. Gandhi, H.T., Vales, M.I., Mallory-Smith, C.A. and Riera-Lizarazu, O. (2009). Genetic structure of Aegilops cylindrica Host in its native range and in the United States of America. Theoretical and Applied Genetics, 119: 1013-25.
8. Gandhi, H.T., Vales, M.I., Watson, C.J.W., Mallory-Smith, C.A., Mori, N., Rehman, M. Zemetra, R.S. and Riera-Lizarazu, O. (2005). Chloroplast and nuclear microsatellite analysis of Aegilops cylindrica. Theoretical and Applied Genetics, 111: 561-72.
9. Goryunova, S.V., Kochieva, E.Z., Chikida, N.N. and Pukhalskyi, V.A. (2004). Phylogenetic relationships and intraspecific variation of D-genome Aegilops L. as revealed by RAPD analysis. Russian Journal of Genetics, 5: 515-23.
10. Haji Karam, M., Naghavi, M.R., Taleii, A.R. and Aghaii, M.J. (2011). Evaluation of Genetic Diversity of Aegilops tauschii from Northern Area of Iran Using SSR Markers. Iranian Journal of Biology, 24: 390-99 (In Persian).
11. Iriki, N., Kawakami, A., Takata, K., Kuwabara, T. and Ban, T. (2001). Screening relatives of wheat for snow mold resistance and freezing tolerance. Euphytica, 122: 335-41.
12. Jaaska, V. (1981). Aspartate aminotransferase and alcohol dehydrogenase isoenzymes: intraspecific differentiation in Aegilops tauschii and the origin of the D genome polyploids in the wheat group. Plant Systematics and Evolution, 137: 259-73.
13. Jam-Baranduzi, A., Sofalian, O., Asghari Zakaria, R., Asghari, A. and Shokrpour, M. (2013). Assessment of genetic diversity in Aegilops species in North-West of Iran using ISSR marker. Yuzuncu Yil University Journal of Agricultural Sciences, 23: 66-75.
14. Johnson, B.L. 1976. Confirmation of the genome donors of Aegilops cylindrica. Nature, 216: 859-62. Khabiri, T., Asghari-Zakaria, R., Zareh, N. and Sofalian, O. (2012). Analysis of genetic diversity based on HMW and LMW glutenin subunits in Aegilops cylindrica from Nourthwest of Iran. International Journal of Agriculture and Crop Sciences, 4: 529-33.
15. Khabiri, T., Asghari Zakaria, R., Zareh, N. and Sofalian, O. (2013). Assessing genetic diversity based on gliadin proteins in Aegilops cylindrica populations from Northwest of Iran. Notulae Scientia Biologicae, 5: 109-13.
16. Kharestani, H. (2010). Assessment of genetic diversity and genomic relationships wild and cultivated wheat species possessing a genome in different ploidy levels using SSR markers, M.Sc. Thesis, Ilam University, Iran (In Persian(.
17. Kumar, S., Tamura, K. and Nei, M. (2004). MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 5: 150-63.
18. Moradkhani, M., Mehrabi, A.A., Etminan, A.R. and Pour Aboughadareh, A.R. (2012). Evaluation of genetic diversity in Aegilops tauschii subspecies from Iran in compare with some wheat with D genome by using of ISSR marker. 12th Iranian Genetics Congress, Shahid Beheshti University International Congress Center, Tehran, Iran.
19. Naghavi, M.R., Aghaei, M.J., Taleei, A.R., Omidi, M., Mozafari, J. and Hassani, M.E. (2009). Genetic diversity of the D-genome in T. aestivum and Aegilops species using SSR markers. Genetic Resources and Crop Evolution, 56: 499-506.
20. Najaphy, A., Ashrafi-Parchin, R. and Farshadfar, E. (2011). Evaluation of genetic diversity in wheat cultivars and breeding lines using Inter Simple Sequence Repeat markers. Biotechnology & Biotechnological Equipment, 25: 2634-38.
21. Peakall, R. and Smouse, P.E. (2006). GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6: 288-95.
22. Perrier, X. and Jacquemoud-Collet, J.P. (2006). DARwin software. Web site at URL: http://darwin.cirad.fr/darwin. Accessed 10 June 2013.
23. Pester, T.A., Ward, S.M., Fenwick, A.L., Westra, P. and Nissen, S.J. (2003). Genetic diversity of jointed goatgrass (Aegilops cylindrica) determined with RAPD and AFLP markers. Weed Science, 51: 287-93.
24. Powell, W., Morgante, M., Ander, C., Hanafey, M., Vogel, J., Tingy, S. and Rafalaski, A. (1996). The comparision of RFLP, RAPD, AFLP and SSR (microsatellite) marker for germplasm analysis. Molecular Breeding, 2: 225-38.
25. Ranjbar, M., Naghavi, M.R., Zali, A.A., Aghaii, M.J., Pirseiedi, M. and Mardi, M. (2008). Evaluation of genetic diversity of Aegilops crassa of Iran using SSR markers. Journal of Genetic Novin, 3: 29-38 (In Persian).
26. Reddy, M.P., Sarla, N. and Siddiq, E.A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9-17.
27. Shiri, M. (2009). Genetic diversity assessment of Einkorn wheat populations of T.boeoticum and T.urartu from West and North-West areas of Iran using SSR markers, M.Sc. Thesis, Ferdowsi University of Mashad, Iran (In Persian).
28. Thomas, K.G. and Bebeli, P.J. (2010). Genetic diversity of Greek Aegilops species using different types of nuclear genome markers. Molecular Phylogenetics and Evolution, 56: 951-961.
29. Terzopoulos, P.J. and Bebeli, P.J. (2008). Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers. Field Crops Research, 108: 39-44.
30. Van-Slageren, M.W. (1994). Wild Wheats: a Monograph of Aegilops L. and Amblyopyrum (jaub. and Spach) Eig (Poaceae), Agricultural University Wageningen, Netherlands.
31. Watanabe, N., Mastui, K. and Furuta, Y. (1994). Uniformity of the alpha-amylase isozymes of Aegilops cylindrica introduced into North American In: Comparisons with ancestral Eurasian accessions (Wang R.R.C., Jensen K.B. and Jaussi, C., Eds.) pp. 215-218, Proc 2 nd Int. Wheat Symp, Logan, UT.
Send email to the article author



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohammadi S, Ashraf Mehrabi A, Arminian A, Fazeli A. Genetic Diversity Structure of Aegilops cylindrica Accessions Revealed by Genomic ISSR Markers. pgr 2014; 1 (1) :13-26
URL: http://pgr.lu.ac.ir/article-1-24-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 1, Issue 1 (2014) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4657