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:: Volume 9, Issue 2 (2023) ::
pgr 2023, 9(2): 31-40 Back to browse issues page
Studying the Expression Pattern of aox2 and pal2 Genes Associated with the Production of Antioxidants and Flavonoids in Yarrow Plant Following Salicylic Acid Treatment
Sodabeh Jahanbakhsh Godehkahriz * , Fatemah Jalali Shahko , Seyedeh Yalda Raisi Sadati
Production Engineering and Plant Genetics Department, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran , jahanbakhsh@uma.ac.ir
Abstract:   (3633 Views)
The various medicinal functions of Yarrow have made it an important medicinal plant in medicine. Also, yarrow is a rich source of antioxidants and flavonoids that protect plants from the harmful effects of active oxygen species. The aim of this study was to investigate the effect of salicylic acid treatment on the expression pattern of two genes (aox2 and pal2) involved in the production of antioxidants and flavonoids in Yarrow plant. For this purpose, a factorial experiment was conducted in a completely randomized design including salicylic acid at two levels (0 and 50 mM) as the first factor and sampling time with two levels (24 and 48 hours) as the second factor in the greenhouse of the Faculty of Agriculture. First, the yarrow plant was subjected to hormonal treatment in Johnson's hydroponic environment, and 24 and 48 hours after the treatment, leaf tissue sampling was done. The results of qRT-PCR showed that salicylic acid stimulation significantly decreased the expression of two genes in the path of producing antioxidants in yarrow. The lowest relative expression of studied genes occurred 48 hours after treatment with salicylic acid. In general, the use of salicylic acid triggers molecular processes that result in the plant responding by changing the expression level of genes encoding antioxidants and flavonoids; therefore, it is suggested to use other stimulants such as jasmonic acid to investigate the expression of genes encoding antioxidants and flavonoids in future researches.
Keywords: Yarrow, Relative gene expression, Hormonal induction
Full-Text [PDF 440 kb]   (985 Downloads)    
Type of Study: Research | Subject: Plant genetics
References
1. Angourani, H.R. (2020). Investigation of phytochemical compounds of yarrow (Achillea millefolium) essential oil in Zanjan province natural habitats. Journal of Medicinal Plant Biotechnology, 6(1): 84-92 (In Persian).
2. Asif, M., Trivedi, P., Solomos, T. and Tucker, M. (2006(. Isolation of high-quality RNA from apple (Malus domestica) Fruit. Journal of Agriculture Food chemistry, 54: 5227-5229. [DOI:10.1021/jf053137n] [PMID]
3. Benedek, B., Rothwangl-Wiltschnigg, K., Rozema, E., Gjoncaj, N., Reznicek, G., Jurenitsch, J., Kopp, B. and Glasl, S. (2008). Yarrow (Achillea millefolium L.) pharmaceutical quality of commercial samples. Journal Pharmazie, 63: 23-26.
4. Bahrami, N., Jalali, M. and Zare, A.A. (2020). The Effect of various sources of Iron on the nitrate accumulation in lettuce (Lactuca sativa L.). Iranian Journal of Soil and Water Research, 51(11): 2953-2963 (In Persian).
5. Butt, U.R., Naz, R., Nosheen, A., Yasmin, H., Keyani, R., Hussain, I. and Hassan, M.N. (2019). Changes in pathogenesis-related gene expression in response to bioformulations in the apoplast of maize leaves against Fusarium oxysporum. Journal of Plant Interactions, 14: 61-72. [DOI:10.1080/17429145.2018.1550217]
6. Bagal, U.R., Leebens mack, J.H., Walter Lorenz, W.W. and Dean, J.F. (2012). The phenylalanine ammonia lyase (PAL) gene family shows a gymnosperm specific lineage. BMC Genomics, 13(3): 1-9. [DOI:10.1186/1471-2164-13-S3-S1] [PMID] [PMCID]
7. Chan, K.L., Ho, C.L., Namasivayam, P. and Napis, S. (2007). A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides. Plant Molecular Biology Group Protocol Exchange. [DOI:10.1038/nprot.2007.184]
8. Elyasi, R., Majdi, M., Bahramnejad, B. and Mirzaghaderi, G.H. (2015). Expression pattern analysis of genes involved in the biosynthetic pathway of monoterpenes and triterpenes in black cumin (Nigella sativa) plants treated with salicylic acid. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23: 164-174 (In Persian).
9. El-Sharkawy, E.E.S., Abdalla, M.Y. and El-Shemy, A.O. (2016). Effect of some resistance chemical inducers on incidence of cucumber Fusarium wilt caused by Fusarium oxysporum f. Sp. cucumerinum. Egyptian Journal of Biological Pest Control, 26: 1-6.
10. Farajollahi, F., Babaezadeh, V. and Rahimian, H. (2018). Induction of several pathogenesis-related genes in tobacco plants treated by salicylic and jasmonic acid after challenging with Pseudomonas syringae pv. Tabaci. Journal of Agriculture Biotechnology, 9: 129-150 (In Persian).
11. Fitzgerald, H.A., Chern, M.S., Navarre, R. and Ronald, P.C. (2004). Overexpression of (At) NPR1 in rice leads to a BTH-and environment-induced lesionmimic/ cell death phenotype. Molecular Plant Microbe Interactions, 17: 140-151. [DOI:10.1094/MPMI.2004.17.2.140] [PMID]
12. Fengqing, W., Jingyu, Z., Zhongyi, Z., Lina, W., Yanfei, S., Caixia, X., Mingjie, L., Bao, Z., Jiafang, D., Li, G. and Hongzheng, S. (2017). Transcriptome analysis of salicylic acid treatment in rehmannia glutinosa hairy roots using RNA-seq technique for identification of genes involved in acteoside biosynthesis. Frontiers in Plant Science, 8: 1-15. [DOI:10.3389/fpls.2017.00787] [PMID] [PMCID]
13. Fathi, I., Majdi, M., Maroufi, A. and Dastan, D. (2020). Expression pattern analysis of some genes involved in the biosynthetic pathway of terpenoids and phenylpropanoids in tissues, developmental stages and under methyl jasmonate treatment in yarrow (Achillea millefolium subsp. millefolium). Journal of Molecular and Cellular Researches (Iranian Journal of Biology), 33: 87-102 (In Persian).
14. Hedayati, V., Mousavi, A., Mousavi, S., Hashemabadi, Z. and Hosseini Mazinani, S.M. (2020). Study on the structure of OeAOX2 gene related to rooting among native olive genotypes (Olea europea L.) of Iran. Journal of Plant Research (Iranian Journal of Biology), 33(2): 496-504 (In Persian).
15. Izadpanah, M., Seyedian, S.E. and Salehi Shanjani, P. (2017). Assessment of agro- morphological diversity among populations of Achillea nobilis L. and Achillea aleppica DC. in Iran. Journal of Plant Research (Iranian Journal of Biology), 29: 702-716 (In Persian).
16. Javedan-Asl, M., Rajabi Memari, H., Nabati Ahmadi, D. and Rahnama Ghahfarokhi, A. (2015). Isolation of linalool synthase and pinene synthase genes from yarrow (Achillea millefolium L.) medicinal plant. Plant Genetic Researches, 2(1): 23-34 (In Persian). [DOI:10.29252/pgr.2.1.23]
17. Jafari Darehdor, A.H., Mohammadabadi, M.R., Esmailizadeh, A.K. and Riahi Madvar, A. (2016). Investigating expression of CIB4 gene in different tissues of Kermani Sheep using Real Time qPCR. Journal of Small Ruminant Research, 4: 119-132 (In Persian).
18. Lei, T., Yan, Y., Xi, D., Sun, H., Zhag, F., Xu, W. and Lin, H. (2008). Effects of Salicylic Acid on alternative pathway respiration and alternative oxidase expression in tobacco calli. Zeitschrift für Naturforschung, 63: 706-712. [DOI:10.1515/znc-2008-9-1016] [PMID]
19. Mahmoodi Jaraghili, P., Mohajjel Shoja, H. and Mohajjel Kazemi, E. (2016). Evaluation of the effect of salinity on the germination and expression of antioxidant genes in two cultivars of tomato plant. Genetic Engineering and Biosafety Journal, 5: 51-60 (In Persian).
20. Mohammadabadi, M.R. and Tohidinejad, F. (2017). Charachteristics determination of Rheb gene and protein in Raini Cashmere goat. Iranian Journal of Applied Animal Science, 7: 289-295 (In Persian).
21. Mora-Pale, M., Sanchez-Rodriguez, S.P. and Linhardt, R.J. (2014). Biochemical strategies for enhancing the in vivo production of natural products with pharmaceutical potential. Current Opinion in Biotechnology, 25: 86-94. [DOI:10.1016/j.copbio.2013.09.009] [PMID]
22. Mirzaei, A., Akbartabar, M., Sadeghi, H. and Sharifi, B. (2010). The Antioxidant activities and total phenolic of Artemisia martima, Achillea millefolium and Matricaria recutica. Armaghane Danesh, 15: 243-252 (In Persian).
23. Pfaffl, M.W., Horgan, G.W. and Dempfle, L. (2002). Relative expression software tool (REST©) for groupwise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30: 1-10. [DOI:10.1093/nar/30.9.e36] [PMID] [PMCID]
24. Poorraskari, O., Nabati Ahmadi, D., Mehdikhanlou, K. and Nejhadsadeghi, L. (2020). Investigating the expression of pinene synthase and linalool genes in response to gibberellic acid in yarrow (Achillea millelium L.) plant. Plant Productions, 43: 13-24 (In Persian).
25. Raman, V. and Ravi, S. (2011). Effect of salicylic acid and methyl jasmonate on antioxidant systems of heamatococcus pluvialis. Acta Physiologiae Plantarum, 33: 1043-1049. [DOI:10.1007/s11738-010-0623-6]
26. Samadi, S., Ghasemnezhad, A. and Alizadeh, M. (2015). Investigation on phenylalanine ammonia-lyase activity of artichoke (Cynara scolymus L.) affected by methyl jasmonate and salicylic acid in in-vitro conditions. Journal of Plant Production Research, 21: 135-148 (In Persian).
27. Taheri, E., Shirzadian-Khorramabad, R., Sharifi-Sirchi, G., Sabouri, A. and Abbaszadeh K. (2016). Assessment of genetic diversity of three yarrow's wild masses in Hormozgan province using morphological traits. Plant Genetic Researches, 2(2): 73-82 (In Persian). [DOI:10.29252/pgr.2.2.73]
28. Upton, R., Graff, A., Jolliffe, G., Länger, R. and Williamson, E. (2016). American Herbal Pharmacopoeia: Botanical Pharmacognosy-Microscopic Characterization of Botanical Medicines. CRC Press, Boca Raton, FL, USA. [DOI:10.1201/b10413]
29. Yakhin, O.I., Lubyanov, A.A., Yakhin, I.A. and Brown, P.H. (2017). Biostimulants in plant science: a global perspective. Front. Plant Science, 7: 20-49. [DOI:10.3389/fpls.2016.02049] [PMID] [PMCID]
30. Yamchi, A. (2015). Sequencing and phylogenetic study of alternative oxidase gene (aox2) in Anthemideae subfamily. Journal of Plant Production Research, 22: 217-229 (In Persian).
31. Ziaei, M., Sharifi, M., Behmanesh, M. and Razavi, K. (2012). Gene expression and activity of phenyl alanine ammonialyase and essential oil composition of Ocimum basilicum L. at different growth stage. Journal of Biotechnology, 10: 32-39 (In Persian).
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Jahanbakhsh Godehkahriz S, Jalali Shahko F, Raisi Sadati S Y. Studying the Expression Pattern of aox2 and pal2 Genes Associated with the Production of Antioxidants and Flavonoids in Yarrow Plant Following Salicylic Acid Treatment. pgr 2023; 9 (2) :31-40
URL: http://pgr.lu.ac.ir/article-1-253-en.html


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Volume 9, Issue 2 (2023) Back to browse issues page
پژوهش های ژنتیک گیاهی Plant Genetic Researches
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