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The Effect of Iron Deficiency on the Expression of Genes Encoding Transcription Factors bZIP4, bZIP79, and bZIP97 in Bread Wheat (Triticum aestivum L.)
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Fatemeh Asadzadeh  , Babak Abdollahi Mandoulakani *  |
| Department of Production Engineering and Plant Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran , b.abdollahi@urmia.ac.ir |
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Abstract: (281 Views) |
To investigate the effect of iron deficiency stress on the expression of genes encoding bZIP4, bZIP79, and bZIP97 transcription factors in iron-efficient and -inefficient bread wheat cultivars, a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of Urmia University. Falat (iron-inefficient) and Pishtaz (iron-efficient) cultivars were grown in iron deficiency and sufficiency conditions. The expression levels of genes mentioned above were measured using real time PCR technique in the leaves and roots of the cultivars at two growth stages: one month after germination (vegetative) and 30% of spiking (reproductive). The results revealed the highest increase in the relative expression of bZIP79 (more than 14-fold change) and bZIP97 (more than 3-fold change) in the leaves of iron-inefficient (Falat) and -efficient (Pishtaz) cultivars, respectively, at vegetative stage. The highest relative expression of bZIP4 was observed in the roots of iron-inefficient cultivars in the vegetative stage. This probably shows that bZIP4 might activate the transcription of the genes responsible for iron uptake from the soil. Increased expression of bZIP79 in the leaves of iron-efficient cultivar in the vegetative stage under iron deficiency conditions, indicates the involvement of this transcription factor in the activation of genes responsible for iron transfer from the leaves to the grain and other tissues. In general, this research helps understand the mechanism of plants coping with iron deficiency stress. Also, the identification of key bZIP transcription factors involved in the activation of genes responsible for iron absorption and transport in bread wheat plants provides the possibility of genetic manipulation of bread wheat cultivars to produce cultivars with a higher amount of iron in the grain.
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| Keywords: bZIP transcription factors, Bread wheat, Iron deficiency, Real time PCR |
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Type of Study: Research |
Subject:
Molecular genetics
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