Introducing Two Stripe Rust Resistance Genes, Yr5 and Yr15 to Some Iranian Bread Wheat Cultivars Using Marker-Assisted Backcrossing

Bagherzadeh, Fatemeh; Mirahmadi, Hannaneh; Pourtabrizi, Soraya; Kazemipour, Ali; Dorraninejad, Maryam; Abdoshahi, Roohollah

doi:‎ 10.22034/PGR.11.1.5

[Home ] [Archive]

[ فارسی ]

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Volume 11, Issue 1 (2024)

pgr 2024, 11(1): 77-88

Back to browse issues page

Introducing Two Stripe Rust Resistance Genes, Yr5 and Yr15 to Some Iranian Bread Wheat Cultivars Using Marker-Assisted Backcrossing

Fatemeh Bagherzadeh

, Hannaneh Mirahmadi

, Soraya Pourtabrizi ^*

, Ali Kazemipour

, Maryam Dorraninejad

, Roohollah Abdoshahi

Department of Plant Production and Genetics, Shahid Bahonar University of Kerman, Kerman, Iran , s.pourtabrizi@empl.uk.ac.ir

Abstract: (574 Views)

Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat in many parts of the world. Genetic resistance is the best way to deal with this disease. This disease evolves very quickly, and for this reason, the resistance of many major-effect resistance genes has been overcome. In this study, the Yr5 and Yr15 genes, which are among the most effective resistance genes in a full-grown plant stage, were introduced to the Iranian cultivars, including Baharan, Rakhshan, Setareh, Sivand, Parsi, and Amin using the marker-assisted backcrossing method. These genes were intrroduced to the mentioned cultivars in six distinct breeding projects. Cultivars were crosses with the donor parents Yr5/6*Avocet‘S’and Yr15/6*Avocet‘S’. The progeny of F₁ generation, were backcrossed with the Iranian cultivars (recurrent parent) to obtain BC₁F₁ progeny in each population. By genotyping 30 random plants in each project, the heterozygous genotypes carrying the resistance genes were identified using specific markers, and the second backcrossing was performed. Overall, the results of this studuy demonstrated that within each population, a stripe rust-resistant line can be developed through repeated generations of backcrossing followed by one generation of selfing. The introduction and pyramiding of yellow rust resistance genes into the Iranian cultivars not only enhance durable resistance and mitigate yield losses during epidemic years but also can contribute to benefits to the environment.

Keywords: Back cross, Durable resistance, Major genes, Resistance genes, Specific marker

Full-Text [PDF 714 kb] (27 Downloads)

Type of Study: Applicable | Subject: Plant improvement

References

1. Aghaei, H., Kasraei, P. and Nasri, M. (2017). The effect of lead and cadmium contamination on germination and grain characteristics of wheat cultivar Sivand. Agricultural Sciences on the Edge of the Desert, 14(1): 23-35 (In Persian).

2. Aktar-Uz-Zaman, M., Tuhina-Khatun, M., Hanafi, M.M. and Sahebi M. (2017). Genetic analysis of rust resistance genes in global wheat cultivars: an overview. Biotechnology & Biotechnological Equipment, 31: 431-445. [DOI:10.1080/13102818.2017.1304180]

3. Barzegari, M., Emam Y. and Zamani, A. (2020). Yield components and grain yield responses of four wheat cultivars to growth retardant Cycocel under terminal drought stress conditions. Journal of Crop Production and Processing, 10(3): 139-156 (In Persian). [DOI:10.47176/jcpp.10.3.20124]

4. Bonnett, D. G., Rebetzke, G. J. and Spielmeyer, W. (2005). Strategies for efficient implementation of molecular markers in wheat breeding. Molecular Breeding, 15: 75-85. [DOI:10.1007/s11032-004-2734-5]

5. Chen, X.M. (2005). Epidemiology and control of strip rust (Puccinia striiformis f. sp. tritici) on wheat. Plant Pathology, 27: 314 -337. [DOI:10.1080/07060660509507230]

6. Ebrahimi, M.N., Ahmadi, H., Darvishnia, M. and Goodarzi, D. (2024). Identification of bread wheat resistant genotypes to fusarium head blight disease and heritability estimation for some traits for bread wheat genotypes. Plant Genetic Researches, 10(2): 137-152 (In Persian). http://dx.doi.org/10.22034/PGR.10.2.1

7. Eskandari Torbeghan, M., Fazli Kakhki, S.F. and Jalini, M. (2023). Compensate for reduced yield due to late water stress by using growth enhancers in the tillering stage of wheat (Triticum aestivum L.). Journal of Plant Environmental Physiology, 70(2): 87-98 (In Persian).

8. Fuchs, M. (2017). Pyramiding resistance-conferring gene sequences in crops. Current Opinion in Virology, 26: 36-42. [DOI:10.1016/j.coviro.2017.07.004]

9. Hospital, F., Moreau, L., Lacoudre, F., Charcosset, A. and Gallais, A. (1997). More on the efficiency of marker-assisted selection. Theoretical and Applied Genetics, 95: 1181-1189. [DOI:10.1007/s001220050679]

10. Hovmoller, M.S. and Justesen, A.F. (2007). Appearance of atypical Puccinia striiformisf. sp. tritici phenotypes in north-western Europe. Australian Journal of Agricultural Research,58: 518-524. [DOI:10.1071/AR06146]

11. Huerta-Espino, J., Singh, R., Crespo-Herrera, L.A. Villaseñor-Mir, H.E., Rodriguez-Garcia, M.F., Dreisigacker, S., Barcenas-Santana, D. and Lagudah, E. (2020). Adult plant slow rusting genes confer high levels of resistance to rusts in bread wheat cultivars from mexico. Frontiers in Plant Science, 11:824 [DOI:10.3389/fpls.2020.00824]

12. Gupta, P.K., Langridge, P. and Mir, R.R. (2010). Marker-assisted wheat breeding: present status and future possibilities. Molecular Breeding, 26: 145-161. [DOI:10.1007/s11032-009-9359-7]

13. Jalili, J., Palash, M. and Jalili, K. (2023). Evaluation of yield, yield components and water use efficiency of wheat cultivar Baharan under cultivation of different irrigation methods in Kermanshah province. Scientific Progress and development of Kermanshah, 2(3): 79-97 (In Persian).

14. Joshi, R.K. and Nayak, S. (2010). Gene pyramiding-A broad spectrum technique for developing durable stress resistance in crops. Microbiology and Molecular Biology Reviews, 5(3): 51-60.

15. Khanmakoo, F., Mohammadi, S.A., Salami, R. and Aharizad, S. (2019). Diversity of Resistance Gene Analogues in Rust Resistance and Susceptible Bread Wheat Varieties. Plant Genetic Researches, 5(2): 29-40 (In Persian). [DOI:10.29252/pgr.5.2.29]

16. Klymiuk, V., Yaniv, E. and Huang, L. (2018). Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family. Nature Communication, 9(1): 3735. [DOI:10.1038/s41467-018-06138-9]

17. Koebner, R.M. and Summers, R.W. (2003). 21st century wheat breeding: plot selection or plate detection?. TRENDS in Biotechnology, 21(2): 59-63. [DOI:10.1016/S0167-7799(02)00036-7]

18. Liu, R., Lu, J. and Zhou, M. (2020). Developing stripe rust resistant wheat (Triticum aestivum L.) lines with gene pyramiding strategy and marker-assisted selection. Genetic Resources and Crop Evolution, 67: 381-391. [DOI:10.1007/s10722-019-00868-5]

19. Liu, R., Lu, J., Zhou, M., Zheng, S., Liu, Z., Zhang, C. and Zhang, L. (2020). Developing stripe rust resistant wheat (Triticum aestivum L.) lines with gene pyramiding strategy and marker-assisted selection. Genetic Resources and Crop Evolution, 67: 381-391. [DOI:10.1007/s10722-019-00868-5]

20. Marchal, C., Zhang, J., Zhang, P., Fenwick, P., Steuernagel, B., Adamski, N.M., Boyd, L., McIntosh, R., Wulff, B.B.H., Berry, S., Lagudah, E. and Uauy, C. (2018). BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust. Nature Plants, 4: 662-668. [DOI:10.1038/s41477-018-0236-4]

21. Najafiyan, G., Amin, H., Afshari, F. and Kabiriyan, H.R. (2010). Parsi, a new bread wheat cultivar, resistant to stem rust (Race Ug99) with good bread making quality for cultivation under irrigated conditions of temperate regions of Iran. Seed and Plant Journal, 26(2): 289-292 (In Persian).

22. Omrani, A., Khodarahmi, M. and Afshari, F. (2016). The evaluation of stripe rust resistance sources in selected wheat genotypes to Puccinia striiformis f. sp. tritici Races. mg.genetics, 4: 547-558 (In Persian).

23. Qie, Y., Liu, Y., Wang, M., Li, X., See, D. R., An, D. and Chen, X. (2019). Development, validation, and re-selection of wheat lines with pyramided genes Yr64 and Yr15 linked on the short arm of chromosome 1B for resistance to stripe rust. Plant disease, 103(1): 51-58. [DOI:10.1094/PDIS-03-18-0470-RE]

24. Rezaie, A., Bijanzadeh, E., Behpouri, A., Barati, V. (2022). Investigation of weed control on assimilate remobilization, yield and yield components in mix cropping of wheat cultivars. Journal of Plant Production Research, 29(2): 1-18 (In Persian).

25. Rosewarne, G.M., Herrera-Foessel, S.A., Singh, R.P., HuertaEspino, J., Lan, C.X. and He, Z.H. (2013). Quantitative trait loci of stripe rust resistance in wheat. Theoretical and Applied Genetics, 126: 2427-2449. [DOI:10.1007/s00122-013-2159-9]

26. Singh, R.P., Huerta Espino, J. and William, H.M. (2005). Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turkish Journal of Agriculture and Forestry, 29: 121-127.

27. Sivasamy, M., Vikas, V.K., Jayaprakash, P., Kumar, J., Saharan, M. S. and Sharma, I. (2017). Gene pyramiding for developing high-yielding disease-resistant wheat varieties. In Management of Wheat and Barley Diseases, 361-409. [DOI:10.1201/9781315207537-13]

28. Sivasamy, M., Vikas, V.K., Jayaprakash, P., Kumar, J., Saharan, M.S. and Sharma, I. (2017). Gene Pyramiding for Developing High-Yielding Disease-Resistant Wheat Varieties. In: Singh, D.P. Ed., Management of Wheat and Barley Diseases. pp. 361-409. Apple Academic Press, Florida, USA. [DOI:10.1201/9781315207537-13]

29. Torabi, M., Madoukhi, V., Nazari, K., Afshari, F., Forootan, A.R., Ramai, M.A., Golzar, H. and Kashani, A.S. (1995). Effectiveness of wheat yellow rust resistance genes in different parts of Iran. Cereal Rusts and Powdery Mildews Bulletin, 23: 9-12 (In Persian).

30. Wan, A.M., Chen, X.M. and He, Z.H. (2007). Wheat stripe rust in China. Australian Journal of Agricultural Research, 58(6): 605-619. [DOI:10.1071/AR06142]

31. Wang, F., Zhang, M., Hu, Y., Gan, M., Jiang, B., Hao, M. and Huang, L. (2023). Pyramiding of adult-plant resistance genes enhances all-stage resistance to wheat stripe rust. Plant Disease, 107(3): 879-885. [DOI:10.1094/PDIS-07-22-1716-RE]

32. Wellings, C.R., Boyd, L.A. and Chen, X.M. )2012(. Resistance to Stripe Rust in Wheat: Pathogen Biology Driving Resistance Breeding. In: Sharma, I., Ed., Disease Resistance in Wheat, pp. 63-83. CABI, Wallingford, UK. [DOI:10.1079/9781845938185.0063]

33. Wellings, C.R. (2007). Puccinia striiformis in Australia: a review of the incursion, evolution, and adaptation of stripe rust in the period 1979-2006. Australian Journal of Agricultural Research, 58: 567-575. [DOI:10.1071/AR07130]

34. Zhang, Y.P., Uyemoto, J. and Kirkpatrick, B. (1998). A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods, 71: 45-50. [DOI:10.1016/S0166-0934(97)00190-0]

35. Zhang, S., Wen, Z., Difonzo, C., Song, Q. and Wang, D. (2018). Pyramiding different aphid-resistance genes in elite soybean germplasm to combat dynamic aphid populations. Molecular Breeding, 38: 1-12. [DOI:10.1007/s11032-018-0790-5]

36. Ziyaev, Z.M., Sharma, R.C., Nazari, K., Morgounov, A.I., Amanov, A.A., Ziyadullaev, Z.F. and Alikulov, S.M. (2011). Improving wheat stripe rust resistance in Central Asia and the Caucasus. Euphytica, 179(1): 197-207. [DOI:10.1007/s10681-010-0305-x]

Send email to the article author

‎ ‎ 10.22034/PGR.11.1.5

Mendeley

Zotero

RefWorks

Bagherzadeh F, Mirahmadi H, Pourtabrizi S, Kazemipour A, Dorraninejad M, Abdoshahi R. Introducing Two Stripe Rust Resistance Genes, Yr5 and Yr15 to Some Iranian Bread Wheat Cultivars Using Marker-Assisted Backcrossing. pgr 2024; 11 (1) :77-88
URL: http://pgr.lu.ac.ir/article-1-309-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 11, Issue 1 (2024)

Back to browse issues page

Persian site map - English site map - Created in 0.08 seconds with 38 queries by YEKTAWEB 4657