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Identification and Expression Analysis of RLP and RLK Gene Family Members in Transcriptome of Saffron Infected with Fusarium Oxysporum Corm Rot
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Fatemeh Hatami   , Farhad Nazarian-Firouzabadi * , Seyed Sajad Sohrabi , Mitra Khademi |
| Production Engineering and Plant Genetic Department, Faculty of Agriculture, Lorestan University, Khorramabad, Iran , khademi.m@lu.ac.ir |
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Abstract: (349 Views) |
Saffron (Crocus sativus L.) is not only one of the most expensive food products in Iran and the world but also holds a special place among Iran's export and industrial products. Since saffron is propagated only through corms, preventing the contamination of corms as “seed” with devastating pathogens is crucial to maintaining the quality and yield of the saffron. Hence, investigating the genetic mechanisms associated with the response of the saffron plant to fungal pathogens such as fusarium wilt rot (Fusarium oxysporum) is of great importance. Given that plants express a wide range of resistance genes to defend themselves, the role of genes related to the PTI (Pattern-Triggered Immunity) pathway, such as those in the LysM-RLK family, is crucial in pathogen resistance. Therefore, studying the transcriptome of saffron corms infected with the pathogen Fusarium oxysporum is important for identifying and investigating the genes belonging to the RLP and RLK gene families. Plants express a wide range of resistance genes to respond to pathogens attacks. Among different gene families associated with PTI pathway, the LysM-RLK family plays an important role in resistance to pathogens. Therefore, in this study, the transcriptome of saffron corms infected with the Fusarium oxysporum was studied to identify and investigate the genes belonging to the RLP and RLK gene families. According to the results of this study, a total of 45 genes encoding PTI pathway receptors were identified in the saffron transcriptome, with 40 sequences belonging to the RLP (Receptor-like proteins) family and 5 sequences to the RLK (Receptor-like kinases) family. The expression analysis of some main RLP and RLK family members showed that the highest expression was related to the sequences of Contig 41583 (RLP) and Contig 61879 (RLK) in the saffron stigma and corms, respectively. Furthermore, the expression of the selected genes in the infected corms significantly increased compared to the control healthy corm. Additionally, the expression levels of the target genes (Contig 41583 and Contig 61879) assessed using qRT-PCR indicated higher expression in corms 72 hours post-infection compared to 48 hours post-inoculation. These results suggest that RLK proteins play a crucial role in the interaction between saffron and the pathogen, particularly due to the presence of the LysM motif. Since LysM motif binds to chitin oligomers of fungal cell walls of certain fungi and oomycetes, it triggers plant immune responses. Overall, the findings of this study are significant for understanding the specific nature of the plant-pathogen relationship and can contribute to insights into the PTI immune pathway. |
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| Keywords: Innate immunity, Chitin, Fusarium, Pattern-Triggered Immunity |
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Full-Text [PDF 964 kb]
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Type of Study: Research |
Subject:
Molecular genetics
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1. Abedi, A., Hajiahmadi, Z., Kordrostami, M., Esmaeel, Q. and Jacquard, C. (2021). Analyses of lysin-motif receptor-like kinase (lysm-rlk) gene family in allotetraploid brassica napus l. and its progenitor species: an in silico study. Cells, 11: 37. [ DOI:10.3390/cells11010037] 2. Antolín-Lovera, M., Ried, M.K., Binder, A. and Parniske, M. (2012). Receptor kinase signaling pathways in plant-microbe interactions. Annual Review of Phytopathology, 50: 451-473. [ DOI:10.1146/annurev-phyto-081211-173002] 3. Bhagat, N., Magotra, S., Gupta, R., Sharma, S., Verma, S., Verma, P.K., Ali, T., Shree, A. and Vakhlu, J. (2022a). Invasion and colonization of pathogenic Fusarium oxysporum R1 in Crocus sativus L. during corm rot disease progression. Journal of Fungi, 8: 1246. [ DOI:10.3390/jof8121246] 4. Bhagat, N., Mansotra, R. and Patel, K. (2022b). Saffron-Fusarium oxysporum R1 dual transcriptomics unravels, defense mechanism of saffron and robust pathogenicity of Fusarium oxysporum R1. [ DOI:10.21203/rs.3.rs-2132821/v1] 5. Cui, H., Tsuda, K. and Parker, J.E. (2015). Effector-triggered immunity: from pathogen perception to robust defense. Annual Review of Plant Biology, 66: 487-511. [ DOI:10.1146/annurev-arplant-050213-040012] 6. Darvishian, A., Nazarian-Firouzabadi, F., Darvishnia, M. and Ismaili, A. (2022). identification of microsatellite molecular markers in saffron (Crocus sativus L.) using RNA-Seq data. Saffron Agronomy and Technology, 10(2): 149-161 (In Persian). 7. Fallahzadeh, V. (2018). Innate immunity in plants. Genetic Engineering and Biosafety Journal, 6: 343-361 (In Persian). 8. Fan, F., Hamada, M., Li, N., Li, G. and Luo, C. (2017). Multiple fungicide resistance in Botrytis cinerea from greenhouse strawberries in Hubei Province, China. Plant Disease, 101: 601-606. [ DOI:10.1094/PDIS-09-16-1227-RE] 9. Frusciante, S., Diretto, G., Bruno, M., Ferrante, P., Pietrella, M., Prado-Cabrero, A., Rubio-Moraga, A., Beyer, P., Gomez-Gomez, L. and Al-Babili, S. (2014). Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis. Proceedings of the National Academy of Sciences, 111: 12246-12251. [ DOI:10.1073/pnas.1404629111] 10. Golmohammadi, F. (2014). Saffron and its farming, economic importance, export, medicinal characteristics and various uses in South Khorasan Province-East of Iran. International Journal of Farming and Allied Sciences, 3: 566-596. 11. Gupta, V., Sharma, A., Rai, P.K., Gupta, S.K., Singh, B., Sharma, S.K., Singh, S.K., Hussain, R., Razdan, V.K. and Kumar, D. (2021). Corm rot of saffron: Epidemiology and management. Agronomy, 11: 339. [ DOI:10.3390/agronomy11020339] 12. Joshi, R. (2018). A review of Fusarium oxysporum on its plant interaction and industrial use. Journal of Medicinal Plants Studies, 6: 112-115. [ DOI:10.22271/plants.2018.v6.i3b.07] 13. Khaledi, N. (2020). Evaluation of cell wall degrading enzymes of Fusarium species associated with root and corm of saffron in South Khorasan province. Saffron Agronomy & Technology 8: 16 (In Persian). 14. Leppyanen, I.V., Pavlova, O.A., Vashurina, M.A., Bovin, A.D., Dolgikh, A.V., Shtark, O.Y., Sendersky, I.V., Dolgikh, V.V., Tikhonovich, I.A. and Dolgikh, E.A. (2021). Lysm receptor-like kinase lyk9 of Pisum Sativum L. may regulate plant responses to chitooligosaccharides differing in structure. International Journal of Molecular Sciences, 22: 711. [ DOI:10.3390/ijms22020711] 15. Li, L., Li, M., Yu, L., Zhou, Z., Liang, X., Liu, Z., Cai, G., Gao, L., Zhang, X. and Wang, Y. (2014). The FLS2-associated kinase BIK1 directly phosphorylates the NADPH oxidase RbohD to control plant immunity. Cell Host & Microbe, 15: 329-338. [ DOI:10.1016/j.chom.2014.02.009] 16. Li, Q., Qi, J., Qin, X., Hu, A., Fu, Y., Chen, S. and He, Y. (2021). Systematic identification of lysin-motif receptor-like kinases (LYKs) in Citrus sinensis, and analysis of their inducible involvements in citrus bacterial canker and phytohormone signaling. Scientia Horticulturae, 276: 109755. [ DOI:10.1016/j.scienta.2020.109755] 17. Lu, Y. and Tsuda, K. (2021). Intimate association of PRR-and NLR-mediated signaling in plant immunity. Molecular Plant-Microbe Interactions, 34: 3-14. [ DOI:10.1094/MPMI-08-20-0239-IA] 18. Luo, J., Zhang, A., Tan, K., Yang, S., Ma, X., Bai, X., Hou, Y. and Bai, J. (2023). Study on the interaction mechanism between Crocus sativus and Fusarium oxysporum based on dual RNA-seq. Plant Cell Reports, 42: 91-106. [ DOI:10.1007/s00299-022-02938-y] 19. Mansotra, R., Ali, T., Bhagat, N. and Vakhlu, J. (2023). Injury and not the pathogen is the primary cause of corm rot in Crocus sativus (saffron). Frontiers in Plant Science, 14: 1074185. [ DOI:10.3389/fpls.2023.1074185] 20. Meng, X. and Zhang, S. (2013). MAPK cascades in plant disease resistance signaling. Annual Review of Phytopathology, 51: 245-266. [ DOI:10.1146/annurev-phyto-082712-102314] 21. Mentis, A.F.A., Dalamaga, M., Lu, C. and Polissiou, M.G. (2021). Saffron for "toning down" COVID-19-related cytokine storm: Hype or hope? A mini-review of current evidence. Metabolism Open, 11: 100111. [ DOI:10.1016/j.metop.2021.100111] 22. Miya, A., Albert, P., Shinya, T., Desaki, Y., Ichimura, K., Shirasu, K., Narusaka, Y., Kawakami, N., Kaku, H. and Shibuya, N. (2007). CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. Proceedings of the National Academy of Sciences, 104: 19613-19618. [ DOI:10.1073/pnas.0705147104] 23. Morkunas, I., Marczak, Ł., Stachowiak, J. and Stobiecki, M. (2005). Sucrose-induced lupine defense against Fusarium oxysporum: Sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to Fusarium oxysporum. Plant Physiology and Biochemistry, 43: 363-373. [ DOI:10.1016/j.plaphy.2005.02.011] 24. Nazarian-Firouzabadi, F., Joshi, S., Xue, H. and Kushalappa, A.C. (2019). Genome-wide in silico identification of LysM-RLK genes in potato (Solanum tuberosum L.). Molecular Biology Reports, 46: 5005-5017. [ DOI:10.1007/s11033-019-04951-z] 25. Nürnberger, T. and Kemmerling, B. (2006). Receptor protein kinases-pattern recognition receptors in plant immunity. Trends in Plant Science, 11: 519-522. [ DOI:10.1016/j.tplants.2006.09.005] 26. Qiu, W., Feechan, A. and Dry, I. (2015). Current understanding of grapevine defense mechanisms against the biotrophic fungus (Erysiphe necator), the causal agent of powdery mildew disease. Horticulture Research, 2: 15020. [ DOI:10.1038/hortres.2015.20] 27. Sattler, S.E. and Funnell-Harris, D.L. (2013). Modifying lignin to improve bioenergy feedstocks: strengthening the barrier against pathogens? Frontiers in Plant Science, 4: 70. [ DOI:10.3389/fpls.2013.00070] 28. Shiu, S.H., Karlowski, W.M., Pan, R., Tzeng, Y.H., Mayer, K.F. and Li, W.H. (2004). Comparative analysis of the receptor-like kinase family in Arabidopsis and rice. The plant cell, 16: 1220-1234. [ DOI:10.1105/tpc.020834] 29. Sohrabi, S.S., Sohrabi, S.M., Mousavi, S.K. and Mohammadi, M. (2020). Identification, sequencing and stability evaluation of eight reference genes in saffron (Crocus sativus L.). Plan Genetic Researches, 7(1): 127-144 (In Persian). [ DOI:10.52547/pgr.7.1.8] 30. Steinkellner, S., Mammerler, R. and Vierheilig, H. (2008). Germination of Fusarium oxysporum in root exudates from tomato plants challenged with different Fusarium oxysporum strains. European Journal of Plant Pathology, 122: 395-401. [ DOI:10.1007/s10658-008-9306-1] 31. Taghikhani, S., Ramshini, H., Sadat-Noori, S.A., Lotfi, M., Izadi Darbakdi, A., Sousaraei, N. and Varvani Farahani, A. (2018). SNP marker assisted selection for identification of fusarium resistant melon plants. Plant Genetic Researches, 5(1): 63-76 (In Persian). [ DOI:10.29252/pgr.5.1.63] 32. Thrane, U. (1990). Grouping Fusarium section Discolor isolates by statistical analysis of quantitative high performance liquid chromatographic data on secondary metabolite production. Journal of Microbiological Methods, 12: 23-39. [ DOI:10.1016/0167-7012(90)90004-P] 33. Tirnaz, S., Bayer, P.E., Inturrisi, F., Zhang, F., Yang, H., Dolatabadian, A., Neik, T.X., Severn-Ellis, A., Patel, D.A. and Ibrahim, M.I. (2020). Resistance gene analogs in the Brassicaceae: Identification, characterization, distribution, and evolution. Plant Physiology, 184: 909-922. [ DOI:10.1104/pp.20.00835] 34. Tombuloglu, G., Tombuloglu, H., Cevik, E. and Sabit, H. (2019). Genome-wide identification of Lysin-Motif Receptor-Like Kinase (LysM-RLK) gene family in Brachypodium distachyon and docking analysis of chitin/LYK binding. Physiological and Molecular Plant Pathology, 106: 217-225. [ DOI:10.1016/j.pmpp.2019.03.002] 35. Williams, H. (1996). Root and stem rot of cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum f. sp. nov. Plant Disease, 80: 313--316. [ DOI:10.1094/PD-80-0313] 36. Yang, H., Bayer, P.E., Tirnaz, S., Edwards, D. and Batley, J. (2020). Genome-wide identification and evolution of receptor-like kinases (RLKs) and receptor like proteins (RLPs) in Brassica juncea. Biology, 10: 17. [ DOI:10.3390/biology10010017] 37. Yu, X., Feng, B., He, P. and Shan, L. (2017). From chaos to harmony: responses and signaling upon microbial pattern recognition. Annual Review of Phytopathology, 55: 109-137. [ DOI:10.1146/annurev-phyto-080516-035649] 38. Zhang, X.C., Wu, X., Findley, S., Wan, J., Libault, M., Nguyen, H.T., Cannon, S.B. and Stacey, G. (2007). Molecular evolution of lysin motif-type receptor-like kinases in plants. Plant Physiology, 144: 623-636. [ DOI:10.1104/pp.107.097097] 39. Zipfel, C. (2014). Plant pattern-recognition receptors. Trends in Immunology, 35: 345-351. [ DOI:10.1016/j.it.2014.05.004] |
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Hatami F, Nazarian-Firouzabadi F, Sohrabi S S, Khademi M. Identification and Expression Analysis of RLP and RLK Gene Family Members in Transcriptome of Saffron Infected with Fusarium Oxysporum Corm Rot. pgr 2024; 11 (1) :121-136 URL: http://pgr.lu.ac.ir/article-1-313-en.html
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