Detection of QTLs Associated to Some Grain Traits in Bread Wheat (Triticum aestivum L.), Using Association Mapping

Mir Drikvand, Reza; Najafian, Goodarz; Bihamta, Mohammad Reza; Ebrahimi, Asa

doi:10.29252/pgr.1.2.43

[Home ] [Archive]

[ فارسی ]

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Volume 1, Issue 2 (2015)

pgr 2015, 1(2): 43-54

Back to browse issues page

Detection of QTLs Associated to Some Grain Traits in Bread Wheat (Triticum aestivum L.), Using Association Mapping

Reza Mir Drikvand ^*

, Goodarz Najafian

, Mohammad Reza Bihamta

, Asa Ebrahimi

Assistant Professor, Department of Agronomy and Plant Breeding, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran , mirderikvand@khoiau.ac.ir

Abstract: (28089 Views)

This study was conducted to identify markers associated with some kernel traits in bread wheat in two separate experiments under field and laboratory. One hundred wheat genotypes were evaluated in an alpha lattice experimental design with two replications. Grain hardness, seed length, seed width and thousand kernel weights were measured. Association mapping was performed based on 96 unlinked and 22 SSR QTL linked markers, using structure and Tassel software. Correction for population structure was performed using genome wide SSR markers so that genotypes were divided into six sub-populations. Totally, 35 SSR markers linked to traits were detected eight of them being QTL linked markers and other markers that were linked to traits, were used to investigate population structure. The QTLs linked markers were as follows: Chromosomes 5B, 5D and 6D had three QTL for grain hardness. Nine QTLs were detected on chromosomes 1A, 1B, 2A, 2B, 2D, 5B, 5D, 6D and 7B for kernel length, kernel width and thousand kernel weights. The results of this study demonstrate that association mapping is a useful approach to complement and enhance previous QTL information for marker-assisted selection in wheat.

Keywords: Kernel traits, Wheat, Association mapping, QTL

Full-Text [PDF 794 kb] (4773 Downloads)

Type of Study: Research | Subject: Plant improvement

References

1. Ammiraju, J.S.S., Dholakia, B.B., Santra, D.K., Singh, H., Lagu, M.D., Tamhankar, S.A., Dhaliwal, H.S., Rao, V.S., Gupta,V.S. and Ranjekar, P.K. (2001). Identification of Inter Simple Sequence Repeat (ISSR) markers associated with seed size in wheat. Theoretical and Applied Genetics, 102: 726-732.

2. Arbelbide, M. and Bernardo, R. (2006). Mixed-model QTL mapping for kernel hardness and dough strength in bread wheat. Theoretical and Applied Genetics, 112: 885-890.

3. Breseghello, F. and Sorrells, M.E. (2006). Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics, 172: 1165-1177.

4. Breseghello, F. and Sorrells, M.E. (2007). QTL analysis of kernel size and shape in two hexaploid wheat mapping populations. Field Crops Research, 101: 172-179.

5. Campbell, K.G., Bergman, C.J., Gualberto, D.G., Anderson, J.A., Giroux, M.J., Hareland, G., Fulcher, R.G., Sorrells, M.E. and Finney, P. L. (1999). Quantitative trait loci associated with kernel traits in a soft ×hard wheat cross. Crop Science, 39: 1184-1195.

6. Cardon, L.R. and Bell, J.I. (2001). Association study designs for complex diseases. Nature Reviews Genetics, 2: 91-99.

7. Committee, A.A.o.C.C.A.M. (2000). Approved Methods of the American Association of Cereal Chemists, 10th edn, AACC, University of Michigan, USA.

8. Crepieux, S., Lebreton, C., Flament, P. and Charmet, G. (2005). Application of a new IBD-based QTL mapping method to common wheat breeding population: analysis of kernel hardness and dough strength. Theoretical and Applied Genetics, 111: 1409-1419.

9. Dholakia, B.B., Ammiraju, J.S.S., Santra, D.K., Singh, H., Katti, M.V., Lagu, M.D., Tmhankar, S.A., Rao., V.S., Gupta, V.S., Dhaliwal, H.S. and Ranjekar, P.K. (2001). Molecular marker analysis of protein content using PCR-Based markers in wheat. Biochemical Genetics, 39: 325-338.

10. Dholakia, B.B., Ammiraju, J.S.S., Singh, H., Lagu, M.D., Röder, M.S. and Rao, V.S, Dhaliwal, H.S., Ranjekar, P.K., Gupta1, V.S., and Weber, W.E. (2003). Molecular marker analysis of kernel size and shape in bread wheat. Plant Breeding, 122: 392-395.

11. Doyle, J.J. and Doyle. J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19:11-15.

12. Galande, A.A., Tiwari, R., Ammiraju, J.S.S., Santra, D.K., Lagu, M.D., Rao, V.S., Gupta, V.S., Misra, B.K., Nagarajan, S. and Ranjekar, P.K. (2001). Genetic analysis of kernel hardness in bread wheat using PCR based markers. Theoretical and Applied Genetics, 103: 601-606.

13. Giroux, M.J. and Morris, C.F. (1997). A glycine to serine change in puroindolineb is associated with wheat grain hardness and low levels of starch-surface friabilin. Theoretical and Applied Genetics, 95: 864-857.

14. Giura, A. and Saulescu, N.N. (1996). Chromosomal location of genes controlling grain size in a large grained selection of wheat (Triticum aestivum L.). Euphytica, 89: 77-80.

15. Igrejas, G., Leroy, P., Charmet, G., Gaborit, T., Marionm, D. and Branlard, G. (2002). Mapping QTLs for grain hardness and puroindoline content in wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 106: 19-27.

16. Jannink, J.L., Bink, M.C. and Jansen, R.C. (2001). Using complex plant pedigrees to map valuable genes. Trends in Plant Science, 6: 337-342.

17. Kumar, N., Kulwal, P., Gaur, A., Tyagi, A., Khurana, J., Khurana, P., Balyan, H. and Gupta, P. (2006). QTL analysis for grain weight in common wheat. Euphytica, 151: 135-144.

18. Kunert, A., Naz, A.A. and Dedeck, O. (2007). AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theoretical and Applied Genetics, 115: 683–695.

19. Lie, Y., Zhou, R., Wang, J., Liao, X., Branlard, G. and Jia, J. (2012). Novel and favorable QTL allele clusters for end-use quality revealed by introgression lines derived from synthetic wheat. Molecular Breeding, 29: 627-643.

20. Marshall, D.R., Mares, D.J., Moss, H.J. and Ellison, F.W. (1986). Effects of grain shape and size on milling yields in wheat. Experimental studies. Australian Journal of Agricultural Research, 37: 342-331.

21. Mattern, P.J., Morris, R., Schmidt, J.W. and Johnson, V.A. (1973). Locations of genes for kernel properties in the wheat variety ‘Cheyenne’ using chromosome substitution lines. In: Agricultural Experimental Station (Sears, E.R, and Sears, L.M.S., Eds) pp. 703-707, Proc 4th international wheat genetics symposium, University of Missouri, Columbia.

22. Narasimhamoorthy, B., Gill, B.S., Fritz, A.K., Nelson, J.C. and Brown-Guedira, G.L. (2006). Advanced backcross QTL analysis of a hard winter wheat × synthetic wheat population. Theoretical and Applied Genetics, 112: 787-796.

23. Oraguzie, N.C. and Wilcox. P.L. (2007). An overview of association mapping. In: Association mapping in plants (Oraguzie, N., Rikkerink, E.A., Gardiner, S. and De Silva, H.N., Eds) pp. 1-9, Springer, New York, USA.

24. Perretant, M.R., Cadalen, T., Charmet, G., Sourdille, P., Nicolas, P., Boeuf, C., Tixier, M.H., Branlard, G., Bernard, S. and Bernard, M. (2000). QTL analysis of bread-making quality in wheat using a doubled haploid population. Theoretical and Applied Genetics, 100: 1167–1175.

25. Pritchard, J.K., Stephens, M., Rosenburg, N.A. and Donnelly, P. (2000). Association mapping in structured populations. American Journal of Human Genetics, 37: 170-181.

26. Pritchard, J.K. and Wen, X. (2007). Documentation for structure Software, version 2.3. Department of Human Genetics, University of Chicago, Chicago, USA.

27. Pritchard, J.K., Wen, X. and Flash, D. (2010). Documentation for structure Software, version 2.3. Department of Human Genetics, University of Chicago, Chicago, USA.

28. Pshenichnikova, T.A., Ermakova, M.F., Chistyakova, A.K., Shchukina, L.V., Berezovskaya, E.V., Lochwasser, U., Röder, M. and Börner, A. (2008). Mapping of the quantitative trait loci (QTL) associated with grain quality characteristics of the bread wheat grown under different environmental conditions. Russian Journal of Genetics, 44: 74-84.

29. Rafalski, J.A., Morgante, M., Powell, W., Vogel, J. M. and Tingey, S.V. (1996). Generating and using DNA markers in plants. In: Analysis of Non-mammalian Genomes: a practical guide (Birren, B. and Lai, E., Eds) pp. 75-134, Academic Press, Boca Raton, Florida, USA.

30. Ramya, P., Chaubal, A., Kulkarni, K, Gipta, L., Kadoo, N., Dhaliwal, H.S., Chhuneja, P., Lagu, M. and Gupta, V. (2010). QTL mapping of 1000-kernel weight, kernel length, and kernel width in bread wheat (Triticum aestivum L.). Journal of Applied Genetics, 51: 421-429.

31. Roder, M.S., Korsun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P. and Ganal, M.W. (1998). (1998). A microsatellite map of wheat. Genetics, 149: 2007-2023.

32. Somers, D.J., Fedak, G, and Savard, M. (2003). Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome, 46: 555-564.

33. Somers, D.J., Isaac, P, and Edwards, K. (2004). A high density microsatellite consensus map of bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109: 1105-1114. Downloaded from journals.lu.ac.ir at 11:06 IRDT on Tuesday September 19th.

34. Sourdille, P., Perretant, M.R., Charmet, G., Leroy, P., Gautier, M.F., Joudrier, P., Nelson, J.C., Sorrells, M.E. and Bernard, M. (1996). Linkage between RFLP markers and genes affecting kernel hardness in wheat. Theoretical and Applied Genetics, 93: 580-586.

35. Toi, J.T., Hareland, G.A., Simsek, S., Chao, S. and Anderson, J.A. (2010). Genome mapping of kernel characteristics in hard red spring wheat breeding lines. Theoretical and Applied Genetics,121: 717–730.

36. Varshney, R.K., Prasad, M., Roy, J.K., Kumar, N., Harjit, S., Dhaliwal, H.S., Balyan, H.S. and Gupta, P.K. (2000). Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTLs for grain weight in bread wheat. Theoretical and Applied Genetics, 100: 1290-1294.

37. Wiersma, J.J., Busch, H., Fulcher, G.G. and Hareland, G. (2001). Recurrent selection for kernel weight in spring wheat. Crop Science, 41: 999-1005.

38. Zhang, Y., Tang, J. and Zhang, Y. (2011). QTL mapping for quantities of protein fractions in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 122: 971-987.

39. Zhu, C., Gore, M.E., Buckler, S. and Yu, J. (2008). Status and prospects of association mapping in plants. The plant genome, 1: 5-20.

Send email to the article author

‎ 10.29252/pgr.1.2.43

‎ 20.1001.1.23831367.1393.1.2.5.2

Mendeley

Zotero

RefWorks

Mir Drikvand R, Najafian G, Bihamta M R, Ebrahimi A. Detection of QTLs Associated to Some Grain Traits in Bread Wheat (Triticum aestivum L.), Using Association Mapping. pgr 2015; 1 (2) :43-54
URL: http://pgr.lu.ac.ir/article-1-35-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 1, Issue 2 (2015)

Back to browse issues page

Persian site map - English site map - Created in 0.07 seconds with 38 queries by YEKTAWEB 4657