Tomato (Solanum Lycopersicum L.) is the second most popular cultivated vegetable in the world. In recent years,
due to the development of the processing industries of agricultural products, the demand for tomatoes has also
significantly increased. With a production of over 3,390,000 tons of tomatoes, Iran ranks 11th in the world for
tomato production. Tomatoes are highly nutritious and have beneficial effects on human health. Understanding the genetic diversity of germplasm is essential for implementing a breeding program aimed at developing highperforming cultivars with improved quality and resistance to biotic and abiotic stresses. Therefore, it is crucial to reveal the genetic diversity in the germplasm of tomato in order to introduce new cultivars. The CBDP marker is one of the emerging molecular markers whose primers are derived from the diversity in the gene-rich regions of plant genomes and with high reproducibility. CBDP marker is easy to use and does not require knowledge of the genome sequence. It is made from the functional regions of the genome and having advantages such as low cost, high polymorphism and extensive genetic information. So, CBDP marker can be useful to assess of the genetic diversity, population structure, genotype identification and QTL mapping. In this research, the genetic diversity of commercial tomato cultivars which are currently or previously being cultivated in Iran in addition to promising lines was assessed via CBDP marker. Additionally, the effectiveness of the CBDP marker system was assessed for investigating the genetic diversity of tomatoes.