Plant breeding has utilized a wide range of techniques and methods to improve the quality and quantity
of plants. The molecular markers are the tools that have provided a new perspective for plant breeding
advancements. This article has reviewed the various advantages and uses of molecular markers and the
utilization of the high potential of natural polymorphisms within communities, combined with the
abilities of conventional plant breeding methods. The marker attributes are not subjected to
environmental influences, and their high frequency number and high structural diversity are as part of
their benefits in identifying identities, determining the genetic diversity of species and studying
relationship between populations. They may aid in discovering more information about protecting and
maintaining genetic stock collections, identifying varieties, determining genes with chromosomal
location and the number of genes controlling traits. Genome sequencing, the preparation of physical and
genetic maps and genomic fingerprinting of plants are some of the other applications of this tool in plant
breeding. The high efficiency of selection with the help of markers in selection of genotypes has been
emphasized as the parent of crosses and selection with the help of a marker in breeding programs and
genomic selection. New technologies offer new opportunities to shape genetic variation in the
improvement of specific plant breeding programs. Nowadays, development of next-generation
sequencing technology, genome sequencing and high throughput approaches for markers have
facilitated EST-derived simple sequence repeat (EST-SSR) marker development as well as single
nucleotide polymorphism (SNP) marker. These markers can be successfully employed in accelerating
research and plant breeding programs.