Plant Genetic Research
Scientific Journal

QTL Mapping of Spike Characteristics in Barley using F 3 and F 4 Families Derived from Badia × Komino Cross

Document Type : Original Article

Authors

Atefeh Kaviani Charati 1
Hossein Sabouri 2
Hossein Ali Fallahi 3
Eisa Jorjani 4

1 Department of Plant Production, Gonbad kavous University, Gonbad kavous, Iran

2 Department of Plant Production, Gonbad kavous University, Gonbad kavous, Irna

3 Department of Agronomic and horticultural Research, Research and Education Center of Agriculture and Natural Resources in Mazandaran, Agricultural Extension and Education Research Organization, Sari, Iran

4 Department of Biology, Gonbad kavous University, Gonbad kavous, Iran

https://doi.org/10.29252/pgr.3.1.13
Abstract
In order to genetic analysis of spike characteristic s in barley , an experiment was conducted with 100 F3 and F 4 barley families derived from Badia × Komino cross at Research Farm college of Agricultural University of Gonbad Kavous (Iran) based on randomized complete block design with three replications. Agronomic traits such as spike length, number of seeds per spike, total of spike, total weight of spike, grain length and grain diameter were measured. Linkage map with 7 SSR and 69 polymorphic alleles of iPBS markers were prepared which covered 632.2 cM of barley genome. QTL analysis was performed based on the method of composite interval mapping (CIM) . Ten QTL s (with additive effect ranged from 127.07 for spike number to -0.625 mm for grain length) were detected. Phenotypic variance explained by QTLs ranged from 10.9 to 12.9 percent, which the highest related to spike length in F 3 generation and the lowest related to the total number of spikes in F 3 generation and the total weight of spike in F4 generation. All detected QTL were major effects and after validation can be used in breeding programs and marker -assisted selection.

Keywords

Barley
Spike characteristic
Molecular markers
Linkage map
QTL
Plant Genetic Research
Volume 3, Issue 1
June 2016
Pages 13-28

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us