Scientific Journal

Assessment of Genetic Diversity Among some Oilseed Rape (Brassica nupus L .) Plants, using Single Sequence Repeats (SSR) Molecular Markers

Document Type : Original Article

Authors

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract
Oilseed rape (Brassica nupus L), a member of Brassicaceae family, is an important crop regarding oil production worldwide. Brassicaceae is an economically important family of flowering plants with about 350 genera and more than 3000 species . Eleven pairs of single sequence repeat (SSR) primers were used to identify the genetic diversity among 21 oilseed rape genotypes . Results of SSR molecular marker analysis revealed that SSR primer s produced a total number of 76 scorable bands of which 46 (60.5%) bands were polymorphics. The average number of bands for each primer and genotype was 6.9 and 3.6, respectively. Both CB10036B and Na10A09 primers produced 10 and Cb10403 primer produced 4 polymorphic bands, respectively. UPGMA cluster analysis based on Dice similarity matrix showed that Zarfam and Gerinimo genotypes had the highest (0.99%) and Licord and KS -11 genotypes had the lowest (0.72 %) similarity. Both Iranian and foreign genotypes were grouped together in one major cluster, indicating presumably they may have the same origin and/or common pedigree . Results of AMOVA analysis within and between groups (spring – Autumn) revealed that almost 97% of total genetic diversity belonged to within group genotypes.

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