Plant Genetic Research
Scientific Journal

Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks

Document Type : Original Article

Authors

Shahin Jahangirzadeh Khiavi 1
Zabihollah Zamani 2
Mohamadreza Fatahi 2
Masoumeh Ashourpour 3

1 Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran

2 Department of Horticulture science, Faculty of Agriculture, University of Tehran, Karaj, Iran

3 Organization of Agriculture - Guilan branch, Rasht, Iran

‎10.29252/pgr.5.1.77
Abstract
The chloroplastic DNA of 64 accessions of apple (Malus spp.) (54 Iranian genotypes, five commercial
cultivars and five rootstocks) were analyzed to reveal their haplotypes by using Polymerase Chain
Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Approximately 4320 bp
of the chloroplast genome was analyzed, by using two chloroplast universal primer pairs and two
restriction enzymes (EcoRI and MseI). All the mutations detected were insertion-deletions been in the
range between 10-40 bp. The combination of all the mutations resulted to six haplotypes (H1, H2, H3,
H4, H5 and H6) in the studied apple accessions. It was defined that the Iranian apple rootstock 'Gami-
Almasi' had a specific haplotype form all the other studied accessions. It was approved that cpDNA
diversity data can be considered for phylogenetic studies in this plant and the polymorphism
determined in cytoplasmic genome by PCR-RFLP method can help to understand the maternal
inheritance in apple.

Keywords

Chloroplast universal primer
Restriction enzyme
Malus Spp
Haplotype
PCR-RFLP
Plant Genetic Research
Volume 5, Issue 1
June 2018
Pages 77-86

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