Plant Genetic Research
Scientific Journal

Identification of Microsatellite Markers Linked with Genomic Regions Involved in Resistance to Basal Stem Rot Disease Isolates in Oily Sunflower (Helianthus annuus L.) under Controlled Conditions

Document Type : Original Article

Authors

Khadijeh Mousa Khalifan 1
Reza Darvishzadeh 2
Masoud Abrinbana 3
Aram Nouri 1

1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

2 Department of Plant Production and Genetics, Urmia University, Urmia, Iran

3 Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran

https://doi.org/10.29252/pgr.5.2.1
Abstract
Sunflower (Helianthus annuus L.) is an important crop that its oil has nutritional and high economic
value. Basal stem rot, caused by Sclerotinia sclerotiorum and S. minor, is one of the important and
devastating disease of sunflower. The use of resistant cultivars is considered as the most important and
effective method to control the disease. In this study, the reaction of 100 oily sunflower lines to three
isolates of S. sclerotiorum and three isolates of S. minor was studied. Identification of gene loci
associated with resistance to disease was done with markers produced with 30 SSR primers pairs. The
results showed that some of sunflower genotypes had well resistant to Sclerotinia disease. Population
structure analysis using Structure software identified 2 subpopulations (K=2). Association analysis
using TASEEL software with general and mixed linear models (GLM and MLM) identified 14 and 12
loci, respectively that have significant association with resistant genes related to Sclerotinia. ORS617
locus was commonly related to genes associated with resistance to M1 from S. minor and J1 from S.
sclerotiorum. The common markers are important in sunflower breeding programs making possible
simultaneously selection for several traits and producing resistant cultivars to Sclerotinia disease.

Keywords

Sunflower
Sclerotinia rot
Linkage disequilibrium
Horizontal resistance
Gene mapping
Plant Genetic Research
Volume 5, Issue 2
December 2018

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