Plant Genetic Research
Scientific Journal

Study on Phylogenetic Relationship among some of Iranian Wild Almond Species using Sequences of ITS1-5.8S rDNA-ITS2 Region and Chloroplastic trnL

Document Type : Original Article

Authors

Seyedeh Zahra Hosseini 1
Behroz Shiran 2
Mohammad Ali Ali Ebrahimi 1

1 Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran

https://doi.org/10.29252/pgr.5.2.17
Abstract
Phylogenetic relations among 12 wild species of almonds, one cultivated almond and one species of
peach were investigated by using of ITS1-5.8S rDNA-ITS2 sequences and trnL region of chloroplast
DNA. To do this, maximum-parsimony and neighbor joining analysis adopted. Results of ITS data
showed that studied species of Prunus only divided in two groups but incapable to separate different
sections. P. tenella showed more diverse genetic distance in compare to other studied species and it
seems that this species does not belong to Amygdalus. Also, by using the ITS data it can be reported
that Prunus is monophyletic. In this research, the genetic distances for each pair of species were
determined and the average genetic distance between species shows only the lowest genetic distance
within the genus. Therefore, Prunus is a single genus. Regarding its high similarity of trnL region in
wild almond species, it can be reported that maternal ancestor of Prunus is the same and trnL is not
optimum marker to separate species of almond.

Keywords

Phylogeny
ITS1-5.8S rDNA-ITS2
Prunus species
trnL chloroplast
Plant Genetic Research
Volume 5, Issue 2
December 2018
Pages 17-28

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