Plant Genetic Research
Scientific Journal

Diversity of Resistance Gene Analogues in Rust Resistance and Susceptible Bread Wheat Varieties

Document Type : Original Article

Authors

Faranak Khanmakoo 1
Seyed Abolghasem Mohammadi 2
Robab Salami 1
Saeed Aharizad 2

1 Department of Plant Breeding and Biotechnology‚ Faculty of Agriculture, Tabriz University, Tabriz, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran

https://doi.org/10.29252/pgr.5.2.29
Abstract
Fungal diseases, especially leaf and stripe rusts are wheat yield reducing factors in Iran and the world.
In this study, genetic diversity of 20 wheat varieties with different response to leaf and stripe rusts
were studied using primers designed based on the conserved regions of plant disease resistance genes.
The banding patterns of polymorphic markers were scored as dominant and number of amplified
bands and percentage of polymorphism were determined. In addition, for each primer combination,
polymorphic information content (PIC) and marker index (MI) were calculated. Out of the 11 used
single primers and primer combinations, five primer combinations and a single primer produced
scorable amplification. The maximum and minimum of PIC were observed for LLOOP-1 and H2016-
H2020 primer combination with mean value of 0.50 and 0.28, respectively. The primer combinations
of H2016-H1146 and H2016-H2020 with mean values of 4.80 and of 2.84, had minimum and
maximum of MI, respectively. Cluster analysis based on Neighbor-Joining algorithm and evolutionary
P-distance coefficient assigned the varieties into four groups which were in agreement with their
response to yellow rust. In principal coordinate analysis, the scatter plot of varieties based on two first
coordinates confirmed the groups obtained from cluster analysis.

Keywords

Resistance gene analogues
Genetic diversity
Yellow rust
Leaf rust
Plant Genetic Research
Volume 5, Issue 2
December 2018
Pages 29-40

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