Plant Genetic Research
Scientific Journal

Comparison of Promoter Sequences of Flowering Control Genes, FT1 and Three Versions of VIN3, in Susceptible and Resistant Sugar Beet Genotypes to Bolting

Document Type : Original Article

Authors

Azadeh Souri 1
Asghar Mirzaie-asl 1
Leila Khodaei 2
Mohammad Reza Abdollahi 3

1 Department of Agricultural Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Department of Agriculture, Payame Noor University, Tehran, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

https://doi.org/10.29252/pgr.6.2.21
Abstract
Autumn sowing of sugar beet is a suitable way in sustainable agriculture. Bolting is an undesirable
phenomenon which reduces sugar beet yield and it is the most important limiting factor in autumn
sowing of sugar beet. Identification and comparison of the sequence of flowering genes in various
genotypes can help to understand the molecular mechanisms controlling bolting. In the previous
studies, it was revealed that expression level of FT1 and VIN3 genes in sugar beet is associated with
bolting resistance. In this study, the sequence of FT1 gene promotor and three versions of VIN3 gene
promoters of sugar beet were compared in three bolting resistant and three bolting susceptible
genotypes. Primer design for each gene was carried out using the DNA sequences found at the NCBI
database. DNA was extracted from leaf samples growing in pots and was used as template in PCR
reactions. Similar length of amplified fragments for each promoter gene in bolting susceptible and
bolting resistant genotypes were selected and sequenced for more accurate assessment. There was no
mutation in the FT1 gene promoter, however 624 substitution and insertion/deletion mutations were
observed in the promoter of three versions of VIN3 gene. A 228-bp ins/del region was detected in the
VIN3-like1 promoter. This region contains promoter elements and seems to have a control function.
Comparison of detected mutations between susceptible and resistant genotypes did not show a distinct
pattern for bolting.

Keywords

Sugar Beet
Bolting
Promotor
Mutation
FT1
VIN3
Plant Genetic Research
Volume 6, Issue 2
December 2019
Pages 21-32

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