Plant Genetic Research
Scientific Journal

Evaluation of MYB93 and MAD8 Genes in Transgenic and Non-Transgenic Rice

Document Type : Original Article

Authors

Kobra Arab
Rudabeh Ravash
Behrouz Shiran

Department of Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekod, Iran

https://doi.org/10.29252/pgr.6.2.33
Abstract
Increasing drought tolerance, especially in rice, which is one of the most important crops in Asia, is
necessary. Transcription factors are specific sequence DNA-binding proteins that are capable of
activating or suppressing transcription. These proteins regulate gene expression levels by binding to
cis regulatory elements in the promoter of target genes to control various biological processes such as
growth, cell division and response to environmental stresses. In this study, MAD8 and MYB93 genes
that were involved in drought stress in rice were considered in two leaf and anther tissues at 0, 24, 48,
72 h and one week after stopping irrigation. The results of q-PCR analysis showed significant
expression changes of these transcription factor genes under drought stress conditions. In this study, a
significant increase in the expression of these genes at 24 h after drought stress in transgenic plants
(Promoter region with accession: NC_029264.1 and GUS gene have transformed) compared to nontransgenic plants showed a relationship between these transcription factors and higher expression of transported promoter in transgenic plants.

Keywords

Rice
Abiotic stress
Transcription factors
MYB93
MAD8
Plant Genetic Research
Volume 6, Issue 2
December 2019
Pages 33-42

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