Anise is an annual plant belonging to Apiaceae family and fruit essential oil of this herb is used in
various pharmaceutical, cosmetic and beverage industries. Drought stress is one of the most critical
limiting factors for anise production in worldwide. In this research, to finding interrelationships among
different traits and performance, some anise genotypes were evaluated using the biplot method. In this
study, 18 anise genotypes were evaluated in normal irrigation regime and drought stress conditions
according to a randomized complete block design with three replications at the field of the Western
Australia University (UWA), Australia. Fifteen phonological, morphological and physiological traits
were measured. Results indicated the positive and significant correlations between yield and fruit
number and fruit thousand weight in both conditions. Due to the negative correlation between
phonological features with fruit yield in two different irrigation conditions, it could be concluded that
to have genotypes with high fruit yield, selection for early ripening genotypes should be done in anise.
In this study, a significant correlation was observed between fruit yield and relative water content in the
stress condition. Therefore, this trait can be used as a physiological index to evaluate drought tolerance
in anise. According to cluster analysis based on the measured traits, genotypes were divided into 3
groups in both non-stress and stress conditions. According to the results of the comparison of the means
of the groups in non-stress and drought stress conditions, genotypes No. 1, 5, 6, 11, 12, 14, 15 and 16
were identified as the most drought-tolerant genotypes. These genotypes could be utilized in breeding
programs for further improvement of drought tolerance in anise germplasm.