Plant Genetic Research
Scientific Journal

Identification and Sequencing of Defensin Genes in Barley (Hordeum vulgare L.): Bioinformatics Analysis and Expression Changes in Response to Biotic and Abiotic Stresses

Document Type : Original Article

Authors

Zeinab Saffar 1
Seyyed Mohsen sohrabi 2
Mohammad Motamedi 3
Anahita Panji 4

1 Department of Plant Biotechnology, Shoushtar Branch, Islamic AzadUniversity, Shoushtar, Iran

2 Department of Production Engineering and Plant Genetics, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Department of Plant Biotechnology, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran

4 Department of Plant Production and Genetic Engineering, Faculty of Agriculture,Lorestan University, Khorramabad, Iran

https://doi.org/10.22034/pgr.2024.11.2.4
Abstract
During evolution, plants have developed diverse mechanisms to cope with biotic and abiotic stresses, one of which is the production and secretion of antimicrobial peptides such as defensins. Plant defensins are short, cysteine-rich and highly stable peptides that have four disulfide bonds in their structures. Defensins, are important group of plant antimicrobial peptides and play vital role in the plant defense system. Barley (Hordeum vulgare L.) due to its importance in agriculture and relative resistance to adverse conditions is a suitable candidate for identifying defensin genes. In the present study, members of the barley defensin gene family were identified and sequenced using bioinformatics and laboratory methods.

Keywords

Antimicrobial peptides
Cysteine-rich peptides
Plant innate immunity
Regulatory gene analysis
Sequencing
Stress-responsive genes
Plant Genetic Research
Volume 11, Issue 2
December 2024
Pages 47-64

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