Scientific Journal

Effect of Trichostatin A Seed Pretreatment on Androgenic Response and Expression of Embryogenesis- and Histone Deacetylase-Related Genes in Anther Cultures of Camelina sativa L.

Document Type : Original Article

Authors

Department of Plant Production and Genetics, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Abstract
The production of doubled haploid plants through in vitro techniques is considered an effective tool to accelerate plant breeding programs and the development of new cultivars. In this regard, Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, has been reported to enhance androgenesis efficiency in some plant species. This study aimed to investigate the effect of TSA on androgenesis response and the expression of certain genes associated with embryogenesis and histone deacetylases in anther culture of Camelina sativa L. To this end, flower buds from seed-grown plants were primed with different concentrations of TSA (0, 0.2, 0.5, 1, and 2 μM), collected at the stage when the anthers contained mid- to late- uninucleate microspores, and then cultured. Subsequently, the relative expression levels of key embryogenesis and HDAC -related genes were evaluated in the resulting calli. Analysis of variance revealed that different TSA treatments significantly (p ≤ 0.05) affected the mean number of embryos per anther, with the highest number of embryos observed at 0.5 and 1 μM TSA treatments (0.417 and 0.483, respectively). Moreover, the relative expression of LEC1, WUS, SERK, BBM, and AGL15 genes increased at these concentrations. In contrast, treatments with 2 μM TSA led to a reduction in the expression level of embryogenesis-related genes and upregulation of HDAC6 and HDAC19 genes. Overall, these findings advance our understanding of complex interaction between chromatin modification and embryogenesis while opening new avenues for exploring epigenetic strategies in plant tissue culture.

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