[صفحه اصلی ]   [Archive] [ English ]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
بخش‌های اصلی
صفحه اصلی::
اطلاعات نشریه::
آرشیو مجله و مقالات::
برای نویسندگان::
برای داوران::
ثبت نام و اشتراک::
تماس با ما::
تسهیلات پایگاه::
بایگانی مقالات زیر چاپ::
فهرست داوران همکار::
::
جستجو در پایگاه

جستجوی پیشرفته
..
دریافت اطلاعات پایگاه
نشانی پست الکترونیک خود را برای دریافت اطلاعات و اخبار پایگاه، در کادر زیر وارد کنید.
..
ISSN
شاپای آنلاین: ISSN 2676-7309
شاپای چاپی: ISSN 2383-1367
..




 
..
:: دوره 7، شماره 1 - ( 1399 ) ::
جلد 7 شماره 1 صفحات 196-181 برگشت به فهرست نسخه ها
مطالعه بیان ژن عامل رونویسی MYB در برخی ارقام گندم نان منطقه سیستان ﺗﺤﺖ ﺷﺮاﻳﻂ ﺗﻨﺶ ﺧﺸﻜﻲ
لیلا فهمیده* ، محمدعلی دلارام پور ، زیبا فولادوند
گروه اصلاح‌نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل؛ دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران ، l.fahmideh@uoz.ac.ir
چکیده:   (7916 مشاهده)
تنش خشکی از جمله تنش‌های غیرزیستی می‌باشد که به‌عنوان یکی از عوامل محدودکننده‌ی منابع غذایی، به‌شمار می‌رود. بروز پاسخ‌های گیاه جهت سازش با شرایط جدید همراه با تغییر الگوی بیانی برخی از ژن‌های عملکردی و تنظیمی می‌باشد. پروتئین‌های MYB یک خانواده بزرگ از عوامل رونویسی هستند که از اهمیت خاصی در تنظیم فرآیندهای نموی و پاسخ‌های دفاعی در گیاهان برخوردارند. این تحقیق با هدف بررسی بیان ژن‌ عامل رونویسی TaMYB73 و فعالیت آنزیم‌های کاتالاز و پلی‌فنل اکسیداز در ارقام گندم‌ نان (هامون، هیرمند، کویر، بولانی و کراس‌بولانی) تحت شرایط تنش خشکی انجام شد. آزمایش فاکتوریل به صورت گلدانی و در قالب طرح بلوک‌های کامل تصادفی با سه تکرار انجام شد. بعد از 45 روز از کشت بذور (مرحله 4 برگی)، تنش خشکی با سطوح مختلف آبیاری انجام شد. بعد از اعمال تنش، نمونه‌برداری از برگ‌‌های گیاهان مورد تیمار جهت سنجش فعالیت آنزیم و بیان ژن صورت گرفت. پس از استخراج RNA و سنتز cDNA، الگوی بیان ژن مورد نظر با استفاده از روش Real-time PCR و تجزیه و تحلیل داده‌ها با استفاده از رابطه CTΔΔ-2 بررسی شد. نتایج نشان داد که سطح بیان ژن TaMYB73 و میزان فعالیت آنزیم‌های کاتالاز و پلی‌فنل پراکسیداز در رقم هیرمند بیشتر از ارقام دیگر بود.
واژه‌های کلیدی: رقم مقاوم، رقم حساس، فنل اکسیداز، کاتالاز، TaMYB73، Real-time PCR
متن کامل [PDF 696 kb]   (1585 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک مولکولی
فهرست منابع
1. Abogadallah, G.M. (2010). Antioxidative defense under salt stress. Plant Signaling and Behavior, 5(4): 369-374 (In Persian).
2. Agarwal, P.K. and Jha, B. (2010). Transcription factors in plants and ABA dependent and independent abiotic stress signaling. Biologia Plantarum, 54: 201-212.
3. Agarwal, S. and Pandey, V. (2004). Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biology Plants, 48: 555-560.
4. Akhtar, W. and Mahmood, T. (2017). Response of rice polyphenol oxidase promoter to drought and salt stress. Pakistan Journal of Botany, 49(1): 29-23.
5. Andarzian, B. (2010). Study and comparison of wheat and Barley Yield under limited Irrigation conditions in Ahwaz climatic conditions. M.Sc. Thesis. Ahvaz University of Shahid Chamran, Ahvaz, Iran (In Persian).
6. Arab, K., Ravash, R. and Shiran, B. (2020). Evaluation of MYB93 and MAD8 genes in transgenic and non-tansgenic Rice. Plant Genetic Researches, 6(2): 33-42 (In Persian).
7. Asadi, A.A., Valizadeh, M., Mohammadi, S.A. and Khodarahmi, M. (2020). Genetic analysis of response to water deficit stress based on physiological traits in Wheat. Plant Genetic Researches, 6(2): 1-20 (In Persian).
8. Beers, G.R. and Sizer, I.V. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195: 133-140.
9. Biehler, K. and Fock, H. (1996). Evidence for the contribution of the Myeloperoxidase reaction in dissipating excess electrons in drought-stressed wheat. Plant Physiology, 112: 265-272.
10. Blum, A., Gozlan, G. and Mayer, J. (1981). The manifestation of dehydration avoidance in wheat breeding germplasm. Crop Science. 21(4): 495-499.
11. Briggle, L.W. and Curtis, B.C. (1987). Wheat worldwide. In Wheat and Wheat Improvement. ASA, CSSA, SSSA, Madison, USA.
12. Cai, H., Tian, S., Dong, H. and Guo, C. (2015). Pleiotropic effects of TaMYB3R1 on plant development and response to osmotic stress in transgenic Arabidopsis. Journal of Gene, 558: 227-234.
13. Chaves, M.S., Martinelli, J.A., Wesp-Guterres, C., Graichen, F.A.S., Brammer, S.P., Scagliusi, S.M. and Consoli, L. (2013). The importance for food security of maintaining rust resistance in wheat. Food Security, 5: 157-176.
14. Dai, X., Xu, Y., Ma, Q., Xu, W., Wang, T., Xue, Y. and Chong, K. (2007). Overexpression of an R1R2R3MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Journal of Plant Physiology, 143: 1739-1751.
15. Dubos, C., Stracke, R., Grotewold, E., Weisshaar, B., Martin, C. and Lepiniec, L. (2010). MYB transcription factors in Arabidopsis. Trends in Plant Science, 15(10): 573-581.
16. Esfandiari, E., Javadi, A., Shokrpour, M. and Shekari, F. (2011). The effect of salt stress on the antioxidant defense mechanisms on wheat seedling. Fresenius Environmental Bulletin, 20(8): 2021-2036.
17. FAO. (2013). FAO Statistical Database (FAOSTAT), Web site at URL: http://www. faostate.fao.org/sit/339/default. aspx. Accessed 09 June 2014
18. Fazeli, F., Ghorbanli, M. and Niknam, V. (2007). Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars. Biologia Plantarum, 51(1): 98-103.
19. Fowler, S. and Thomashow, M.F. (2002). Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell, 14: 1675-1690.
20. Gharbi, A., Rashidin, A., Tarynzhad, S. and Chlbyyany, Q. (2013). Salinity and drought tolerance of durum wheat lines under greenhouse conditions. Journal of Crop Ecophysiology, 4(28): 393-410 (In Persian).
21. Ghasemi Pirbalouti, A., Samani, M.R., Hashemi, M. and Zeinali, H. (2014). Salicylic acid affects growth, essential oil and chemical compositions of thyme (Thymus daenensis Celak.) under reduced irrigation. Plant Growth Regulation, 72: 289-301.
22. Gressel, J., Galun, E., Foyer, C.H. and Mullineaux, P.M. (1994). Genetic Controls of Photooxidant Tolerance. CRC Press Inc. Boca Raton USA.
23. Hernandez, J.A., Jimenez, A., Mullineaux, P. and Sevilla, F. (2000). Tolerance of pea (Pisumsativum L.) to long term salt stress is associated with induction of antioxidant defenses. Plant Cell Environment, 23: 853-862.
24. Hong, W. and Ji-Yan, J. (2007). Effects of zinc deficiency and drought stress on plant growth and metabolism of reactive oxygen species in maize (Zea mays L.). Agricultural Science in China, 6(8): 988 -995.
25. Javadi, S.M., Shabr, Z.S., Pour Abed, A. and Ghadiri, S. (2016). Reconstruction and analysis of drought tolerance gene networks in Hordeum vulgare leaf. The First International Conference and the 9th National Biotechnology Conference of the Islamic Republic of Iran. May 3rd to 5th, Tehran (In Persian).
26. Kar, M. and Mishra, D. (1976). Catalase, peroxidase, and polyphenol oxidase activities during rice leaf senescence. Plant Physiology, 57(2): 315-319.
27. Khazaei, B. (2002). Effect of drought stress on yield and physiological characteristic of resistant and susceptible wheat cultivars and introduction of the most appropriate drought resistance indices. Ph.D. Thesis, Ferdowsi University, Mashhad, Iran (In Persian).
28. Kouchaki, A. and Alizadhe, A. (1986). Crop Production in Dry Regions. Astan Qods Razavi Press, Mashhad, IR (In Persian).
29. Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real time quantitative PCR and the 2ΔΔ(CT) method. Methods, 25(4): 402-408.
30. Naeemi, T., Fahmideh, L. and Fakheri, B. (2019). The effect of drought stress on MYB gene expression and osmotic regulator levels of five durum wheat genotypes (Triticum turgidum L.). Nova Biologica Reperta, 6: 217-228 (In Persian).
31. Naeemi, T., Fahmideh, L. and Fakheri, B.A. (2018). The impact of drought stress on antioxidant enzymes activities, containing of proline and carbohydrate in some genotypes of durum wheat (Triticum turgidum L.) at seedling stage. Journal of Crop Breeding, 10(26): 22-31 (In Persian).
32. Naghavi, M.R. and Mardi, M. (2010). Characterization of genetic variation among accessions of Aegilops tauschii. Journal of Molecular Biology Biotechnology, 18: 93-96.
33. Raesi, R., Fakheri, B. and Mahdinezhad, N. (2019). Evaluation of the effect of Glomus fascollaria on some morphological characteristics, photosynthetic pigments and antioxidant activity of Chicory (Cichorium intybus L.) under drought stress. Environmental Stresses in Crop Sciences, 12(2): 459-505.
34. Rahaei, M., Xue, G.P., Naghavi, M.R., Alizadeh, H. and Schenk, P.M. (2010). A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Reports, 29: 835-844.
35. Reddy, A.R., Chaitanya, K.V. and Vivekanandan, M. (2004). Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161(11): 1189-1202.‌
36. Riechmann, J.L., Heard, J., Martin, G., Reuber, L., Jiang, C.Z., Keddie, J., Adam, L., Pineda, O., Ratcliffe, O.J., Samaha, R.R., Creelman, R., Pilgrim, M., Broun, P., Zhang, J.Z., Ghandehari, D., Sherman, B.K. and Yu, G.L. (2000). Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 290: 2105 -2110.
37. Sairam, R.K. and Saxena G.C. (2000). Oxidative stress and antioxidant in wheat genotype: possible mechanism of water stress tolerance. Agronomy and Crop Sciences, 184(1): 55-61.
38. Sairam, R.K., Rao, K.V. and Srivastava. G.C. (2002). Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163: 1037-1046.
39. Shahriari, P., Mirshams Kakhaki, A. and Amini, M. (2012). Molecular detection of enzyme polyphenol oxidase in a number of common wheat varieties in Iran marker by STS. Iran's Third National Conference on Agricultural Biotechnology of Plant, Animal and Industrial. Mashhad, Ferdowsi University of Mashhad, Mashhad, Iran (In Persian).
40. Shehab, G.G., Ahmed, O.K. and El-Beltagi, H.S. (2010). Effects of various chemical agents for alleviation of drought stress in rice plants (Oryza sativa L). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38: 139-148.
41. Stracke, R., Werber, M. and Weisshaar, B. (2001). The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Biotechnology, 4: 447-456.
42. Tabaraki, H., Fahmideh, L. and Fooladvand, Z. (2017). Study of MYB gene expression under drought stress in some bread wheat cultivars. Journal of Genetic Engineering and Biosafety, 6: 95-104 (In Persian).
43. Tarahomi, P., Lahooti, D. and Abbasi, P. (2010). Effects of drought stress on soluble sugars, chlorophyll and potassium S. leriifolia (Salvia leriifolia Benth). Journal of Biological Sciences, 3(2): 1-7 (In Persian).
44. Thipyapong, P., Melkonian, J., Wolfe, D.W. and Steffens, J.C. (2004). Suppression of polyphenol oxidases increases stress tolerance in tomato. Plant Science, 167(4): 693-703.
45. Thirumalaikumar, V.P., Devkar, V., Mehterov, N., Ali, S., Ozgur, R., Turkan, I. and Balazadeh, S. (2018). NAC transcription factor JU NGBRUNNEN 1 enhances drought tolerance in tomato. Plant Biotechnology Journal, 16: 354-366.
46. Umezawa, T., Fujita, M., Fujita, Y., Yamaguchi-Shinozoki, K. and Shinozoki, K. (2006). Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future. Current Opinion in Biotechnology, 17: 113-122.
47. Vaughn, K.C., Lax, A.R. and Duke, S.O. (1988). Polyphenol oxidase: the chloroplast oxidase with no established function. Physiologia Plantarum, 72: 659-665.
48. Yar Hussain, M., Heydariyan, Z., Neazaei, A. and Ramezani, L. (2011). Examined the expression levels of two genes encoding the transcription factors related-MYB family in two varieties of wheat under salt stress. National Biotechnology Congress of Islamic Republic of Iran, Tehran, Iran (In Persian).
49. Yoshimura, K., Yabute, Y., Ishikawa, T. and Shigeoka, S. (2000). Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiology, 123: 223-233.
50. Zamani, Z., Niakan, M. and Ghorban-Ali, M. (2013). Influence of exogenous putrescine on phenolic compounds, antioxidant enzymes activity and nitrate reductase in seed germination under drought stress. Journal of Iranian Plant Ecophysiological Research, 8(3): 78-90.
51. Zhang, L., Liu, G., Zhao, G., Xia, C., Jia, J., Liu, X. and Kong, X. (2014). Characterization of a wheat R2R3-MYB transcription factor gene, TaMYB19, involved in enhanced abiotic stresses in Arabidopsis. Plant and Cell Physiology, 55: 1802-1812.
ارسال پیام به نویسنده مسئول



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fahmideh L, Delarampoor M A, Fooladvand Z. Study of MYB Transcription Factor Gene Expression in Some Bread Wheat Cultivars of Sistan Region, Iran. pgr 2020; 7 (1) :181-196
URL: http://pgr.lu.ac.ir/article-1-168-fa.html

فهمیده لیلا، دلارام پور محمدعلی، فولادوند زیبا. مطالعه بیان ژن عامل رونویسی MYB در برخی ارقام گندم نان منطقه سیستان ﺗﺤﺖ ﺷﺮاﻳﻂ ﺗﻨﺶ ﺧﺸﻜﻲ. پژوهش های ژنتیک گیاهی. 1399; 7 (1) :181-196

URL: http://pgr.lu.ac.ir/article-1-168-fa.html



بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.
دوره 7، شماره 1 - ( 1399 ) برگشت به فهرست نسخه ها
پژوهش های ژنتیک گیاهی Plant Genetic Researches
Persian site map - English site map - Created in 0.08 seconds with 40 queries by YEKTAWEB 4657