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:: دوره 7، شماره 1 - ( 1399 ) ::
جلد 7 شماره 1 صفحات 196-181 برگشت به فهرست نسخه ها
مطالعه بیان ژن عامل رونویسی MYB در برخی ارقام گندم نان منطقه سیستان ﺗﺤﺖ ﺷﺮاﻳﻂ ﺗﻨﺶ ﺧﺸﻜﻲ
لیلا فهمیده* ، محمدعلی دلارام پور ، زیبا فولادوند
گروه اصلاح‌نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل؛ دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران ، l.fahmideh@uoz.ac.ir
چکیده:   (7727 مشاهده)
تنش خشکی از جمله تنش‌های غیرزیستی می‌باشد که به‌عنوان یکی از عوامل محدودکننده‌ی منابع غذایی، به‌شمار می‌رود. بروز پاسخ‌های گیاه جهت سازش با شرایط جدید همراه با تغییر الگوی بیانی برخی از ژن‌های عملکردی و تنظیمی می‌باشد. پروتئین‌های MYB یک خانواده بزرگ از عوامل رونویسی هستند که از اهمیت خاصی در تنظیم فرآیندهای نموی و پاسخ‌های دفاعی در گیاهان برخوردارند. این تحقیق با هدف بررسی بیان ژن‌ عامل رونویسی TaMYB73 و فعالیت آنزیم‌های کاتالاز و پلی‌فنل اکسیداز در ارقام گندم‌ نان (هامون، هیرمند، کویر، بولانی و کراس‌بولانی) تحت شرایط تنش خشکی انجام شد. آزمایش فاکتوریل به صورت گلدانی و در قالب طرح بلوک‌های کامل تصادفی با سه تکرار انجام شد. بعد از 45 روز از کشت بذور (مرحله 4 برگی)، تنش خشکی با سطوح مختلف آبیاری انجام شد. بعد از اعمال تنش، نمونه‌برداری از برگ‌‌های گیاهان مورد تیمار جهت سنجش فعالیت آنزیم و بیان ژن صورت گرفت. پس از استخراج RNA و سنتز cDNA، الگوی بیان ژن مورد نظر با استفاده از روش Real-time PCR و تجزیه و تحلیل داده‌ها با استفاده از رابطه CTΔΔ-2 بررسی شد. نتایج نشان داد که سطح بیان ژن TaMYB73 و میزان فعالیت آنزیم‌های کاتالاز و پلی‌فنل پراکسیداز در رقم هیرمند بیشتر از ارقام دیگر بود.
واژه‌های کلیدی: رقم مقاوم، رقم حساس، فنل اکسیداز، کاتالاز، TaMYB73، Real-time PCR
متن کامل [PDF 696 kb]   (1523 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: ژنتیک مولکولی
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Fahmideh L, Delarampoor M A, Fooladvand Z. Study of MYB Transcription Factor Gene Expression in Some Bread Wheat Cultivars of Sistan Region, Iran. pgr 2020; 7 (1) :181-196
URL: http://pgr.lu.ac.ir/article-1-168-fa.html

فهمیده لیلا، دلارام پور محمدعلی، فولادوند زیبا. مطالعه بیان ژن عامل رونویسی MYB در برخی ارقام گندم نان منطقه سیستان ﺗﺤﺖ ﺷﺮاﻳﻂ ﺗﻨﺶ ﺧﺸﻜﻲ. پژوهش های ژنتیک گیاهی. 1399; 7 (1) :181-196

URL: http://pgr.lu.ac.ir/article-1-168-fa.html



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