اثر پیش‌تیمار بذر با تریکواستاتین A بر پاسخ به آندروژنز و بیان ژن‌های مرتبط با رویان‌زایی و هیستون داستیلاز در کشت بساک گیاه کاملینا (.Camelina sativa L)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه بو علی سینا، همدان، ایران

چکیده
تولید گیاهان هاپلوئید مضاعف از طریق روش‌های درون‌شیشه‌ای، ابزاری مؤثر برای تسریع برنامه‌های به­نژادی و تولید ارقام جدید محسوب می‌شود. در این راستا، تریکوستاتین  A(TSA) به­عنوان بازدارنده آنزیم‌های هیستون‌داستیلاز (HDAC)، در برخی گیاهان موجب افزایش کارایی آندروژنز شده است. این پژوهش با هدف بررسی اثر TSA بر پاسخ آندروژنز و بیان برخی ژن‌های مرتبط با رویان‌زایی و هیستون‌داستیلاز در کشت بساک گیاه کاملینای زراعی انجام شد. بدین منظور، غنچه‌های گیاهان حاصل از بذور تیمارشده با غلظت‌های مختلف TSA (صفر، 2/0، 5/0، ۱ و ۲ میکرومول) در مرحله‌ای که بساک‌ها حاوی میکروسپورهای تک‌هسته‌ای میانی تا انتهایی بودند، برداشت و کشت شدند. در ادامه، بیان نسبی ژن‌های کلیدی رویان‌زایی و HDAC در کالوس‌های حاصل ارزیابی شد. نتایج تجزیه واریانس داده­ها، نشان داد که تیمارهای مختلف TSA در سطح 05/0 اثر معنی‌داری بر میانگین تعداد رویان به‌ازای هر بساک داشتند، به‌طوری‌که بیشترین تعداد رویان در تیمارهای ۱ و 5/0 میکرومول (به‌ترتیب 483/0 و 417/0) مشاهده شد. همچنین، بیان نسبی ژن‌های LEC1، WUS، SERK، BBM و AGL15 در غلظت‌های مذکور افزایش یافت. در مقابل، تیمار ۲ میکرومول TSA باعث کاهش بیان ژن‌های رویان‌زایی و افزایش بیان ژن‌های HDAC6 و HDAC19 شد. به‌طور کلی یافته­های این پژوهش، اطلاعات و شناخت محققین از برهم‌کنش پیچیده بین تغییر ساختار کروماتین و فرآیندهای رویان‌زایی را افزایش می­دهد و فرصت‌های جدیدی برای مطالعه استراتژی‌های اپی‌ژنتیکی در کشت‌بافت گیاهی فراهم می‌کند. 

کلیدواژه‌ها

موضوعات


Abrahamsson, M., Valladares, S., Merino, I., Larsson, E. and von Arnold, S. (2017). Degeneration pattern in somatic embryos of Pinus sylvestris L. In Vitro Cellular & Developmental Biology Plant, 53: 86–96. https://doi.org/10.1007/s11627-016-9797-y
Ahmad, M., Waraich, E.A., Hafeez, M.B., Zulfiqar, U., Ahmad, Z. and Iqbal, M.A. (2022). Changing Climate Scenario: Perspectives of Camelina sativa as Low-Input Biofuel and Oilseed Crop. In: Ahmed, M., Ed., Global Agricultural Production: Resilience to Climate Change, pp. 197–236. Springer Cham, Gewerbestrasse, CH. https://doi.org/10.1007/978-3-031-14973-3_7
Arshad, M.K., Mohanty, A., Acker, R.V., Riddle, R., Todd, J. and Khalil, H. (2022). Valorization of camelina oil to biobased materials and biofuels for new industrial uses: A review. Royal Society of Chemistry Advances, 12(43): 27230–27245. https://doi.org/10.1039/D2RA03253H
Azizian Mosleh, R., Abdollahi, M.R., Sarikhani, H., Mirzaie-Asl, A. and Pour Mohammadi, P. (2021) Study of the effect of 5-Azacytidine as a DNA demethylating agent on agronomic traits, androgenesis induction via anther culture and DNA-methyltransferase gene expression in maize (Zea mays L.) leaf tissue. Plant Genetic Research, 7(2): 119-134 (In Persian). https://doi.org/10.52547/pgr.7.2.10
Bannister, A.J. and Kouzarides, T. (2011) Regulation of chromatin by histone modifications. Cell Research, 21(3): 381–395. https://doi.org/10.1038/cr.2011.22
Bie, X.M., Dong, L. and Li, X.H. (2020). Trichostatin A and sodium butyrate promotes plant regeneration in common wheat. Plant Signaling and Behavior, 15:1820681. https://doi.org/10.1080/15592324.2020.1820681
Blume, R.Y., Kalendar, R., Guo, L., Cahoon, E.B. and Blume, Y.B. (2023). Overcoming genetic paucity of camelina sativa: possibilities for interspecific hybridization conditioned by the genus evolution pathway. Frontiers in Plant Science, 14: 1259431. https://doi.org/10.3389/fpls.2023.1259431
Boutilier, K., Offringa, R., Sharma, V.K., Kieft, H., Ouellet, T., Zhang, L., Hattori, J., Liu, C. M., Van Lammeren, A.A.M. and Miki, B.L.A. (2002). Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. The Plant Cell, 14: 1737–1749. https://doi.org/10.1105/tpc.001941
Chang, S. and Pikaard, C.S. (2005). Transcript profiling in Arabidopsis reveals complex responses to global inhibition of DNA methylation and histone deacetylation. Journal of Biological Chemistry, 280: 796–804. https://doi.org/10.1074/jbc.M409053200
Chaudhary, R., Koh, C.S., Kagale, S., Tang, L., Wu, S.W. and Lv, Z. (2020). Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa. G3: Genes| Genomes| Genetics, 10(4): 1297-1308. https://doi.org/10.1534/g3.119.400957
Chao, W., Wang, H., Horvath, D. and Anderson, J. (2019).  Selection of endogenous reference genes for qRT-PCR analysis in Camelina sativa and identification of FLOWERING LOCUS C allele-specific markers to differentiate summer- and winter-biotypes. Industrial Crops and Products, 129:495–502. https://doi.org/10.1016/j.indcrop.2018.12.017
Choi, S.H., Ahn, W.S., Lee, M.H., Jin, D.M., Lee, A., Jie, E.Y., Ju, S.J., Ahn, S.J. and Kim, S.W. (2023) Effects of TSA, NaB, Aza in Lactuca sativa L. proto­plasts and effect of TSA in Nicotiana Benthamiana protoplasts on cell division and callus formation. PLoS ONE, 24(2): e0279627. https://doi.org/10.1371/journal.pone.0279627
Custers, J.B.M. (2003). Microspore culture in crop plants. In: Maluszynski, M., Kasha, K.J., Forster, B.P. and Szarejko, I. Eds., Doubled Haploid Production in Crop Plants, pp. 185–199. Springer Dordrecht, Dordrecht, NL. https://doi.org/10.1007/978-94-017-1293-4_29
Fallah, F., Kahrizi, D., Rezaeizad, A., Zebarzadi, A, and Zarei, L. (2020). Evaluation of genetic variation and parameters of fatty acid profile in doubled haploid lines of Camelina sativa L.. Plant Genetic Research, 6(2): 79-96 (In Persian). https://doi.org/10.29252/pgr.6.2.79
Feng, W. and Michaels, S.D. (2015). Accessing the inaccessible: The organization, transcription, replication, and repair of heterochromatin in plants. Annual Review of Genetics, 49: 439–459. https://doi.org/10.1146/annurev-genet-112414-055048
Feng, S., Ma, C., Wang, J. and Li, Y. (2017). Histone modifications in plant abiotic stress responses. Plant Science, 8: 2038.
Ferrie, A.M. and Caswell, K.L. (2011). Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue and Organ Culture, 104(3): 301–309. https://doi.org/10.1007/s11240-010-9800-y
Fletcher, R. Coventry, J. and Kott, L.S. (1998). Doubled Haploid Technology for Spring and Winter Brassica napus. Technical Bulletin OAC Publication, CA.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Nutrient requirement of suspension culture of soybean root cells. Experimental Cell Research, 50: 151–158. https://doi.org/10.1016/0014-4827(68)90403-5
Görisch, S.M., Wachsmuth, M., Toth, K.F., Lichter, P. and Rippe, K. (2005). Histone acetylation increases chromatin accessibility. Journal of Cell Science, 118: 5825–5834. https://doi.org/10.1242/jcs.02689
Han, L., Silvestre, S., Sayanova, O., Haslam, R.P. and Napier, J.A. (2022). Using field evaluation and systematic iteration to rationalize the accumulation of omega‐3 long‐chain polyunsaturated fatty acids in transgenic Camelina sativa. Plant Biotechnology Journal, 20(10): 1833-1845. https://doi.org/10.1111/pbi.13867
Inoue, K., Oikawa, M., Kamimura, S., Ogonuki, N., Nakamura, T. and Nakano, T. (2015). Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer. Scientific Reports, 5: 10127. https://doi.org/10.1038/srep10127
Jeong, M.J., Kim, M.H., Kim, K., Park, C. M. and Kim, J.H. (2024). Trichostatin A promotes de novo shoot regeneration from Arabidopsis callus via the cytokinin pathway. Scientific Reports, 14(1): 1074.
Jiang, F., Ryabova, D., Diedhiou, J., Hucl, P., Randhawa, H., Marillia, E.F., Foroud, N.A., Eudes, F. and Kathiria, P. (2017). Trichostatin A increases embryo and green plant regeneration in wheat. Plant Cell Reports, 36: 1701–1706. https://doi.org/10.1007/s00299-017-2183-3
Kagale, S., Warkentin, T.D. and Kagale, S. (2012). Efficient doubled haploid production in Camelina sativa (L.) Crantz via isolated microspore culture. Plant Cell Reports, 31(1): 185-194.
Li, H., Soriano, M., Cordewener, J.H. G., Muiño, J.M., Riksen‑Bruinsma, T., Fukuoka, H., Angenent, G.C. and Boutilier, K.A. (2014). The histone deacetylase inhibitor trichostatin A promotes totipotency in the male gametophyte. The Plant Cell, 26:195–209. https://doi.org/10.1105/tpc.113.116491
Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆ct method. Methods, 25(4): 402–408. https://doi.org/10.1006/meth.2001.1262
Marks, P.A., Richon, V.M. and Rifkind, R.A. (2000). Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells. Journal of the National Cancer Institute, 92(15):1210–1216. https://doi.org/10.1093/jnci/92.15.1210
Martinez, Ó., Arjones, V., González, M.V. and Rey, M. (2021) Histone deacetylase inhibitors increase the embryogenic potential and alter the expression of embryogenesis-related and hdac-encoding genes in grapevine (Vitis vinifera L., cv. mencía). Plants, 10: 1164–1183. https://doi.org/10.3390/plants10061164
Moronczyk, J., Braszewska, A. and Wójcikowska, B. (2022). Insights into the histone acetylation-mediated regulation of the transcription factor genes that control the embryogenic transition in the somatic cells of Arabidopsis. Cells, 11: 863. https://doi.org/10.3390/cells11050863
Nowak, K., Wójcikowska, B., Gajecka, M., Elżbieciak, A., Morończyk, J., Wójcik, A., Żemła, P., Citerne, S., Kiwior‑Wesołowska, A., Zbieszczyk, J. and Gaj, M. (2024). The improvement of the in vitro plant regeneration in barley with the epigenetic modifer of histone acetylation, trichostatin A. Plant Genetics Original Paper, 65(1): 13-30. https://doi.org/10.1007/s13353-023-00800-9
Tanaka, M., Kikuchi, A. and Kamada, H. (2008). The Arabidopsis histone deacetylases HDA6 and HDA19 contribute to the repression of embryonic properties after germination. Plant Physiology, 146:149–161. https://doi.org/10.1104/pp.107.111674
Tsuwamoto, R., Yokoi, S. and Takahata, Y. (2010). Arabidopsis EMBRYOMAKER encoding an AP2 domain transcription factor plays a key role in developmental change from vegetative to embryonic phase. Plant Molecular Biology, 73: 481–492. https://doi.org/10.1007/s11103-010-9634-3
Valero-Rubira, I., Castillo, A.M., Burrell, M.Á. and Vallés, M.P. (2023). Micro­spore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expres­sion in bread wheat. Frontiers in Plant Science, 13: 1058421. https://doi.org/10.3389/fpls.2022.1058421
Wang, X., Niu, Q.W. and Teng, C. (2009). Overexpression of PGA37/ MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis. Cell Research, 19: 224–235. https://doi.org/10.1038/cr.2008.276
Wang, H.M., Enns, J.L., Nelson, K.L., Brost, J.M., Orr, T.D. and Ferrie, A.M.R. (2019). Improving the efficiency of wheat microspore culture methodology: Evaluation of pretreatments, gradients, and epigenetic chemicals. Plant Cell Tissue Organ Culture. 139: 589–599. https://doi.org/10.1007/s11240-019-01704-5
Wójcikowska, B., Botor, M., Moronczyk, J., Wójcik, A.M., Nodzynski, T., Karcz, J. and Gaj, M.D. (2018). Trichostatin A triggers an embryogenic transition in Arabidopsis explants via an auxin-related pathway. Frontiers in Plant Science. 9: 1353. https://doi.org/10.3389/fpls.2018.01353
Xu, L., Zhao, H., Liu, W. and Gao, F. (2017). Histone modification in plant somatic embryogenesis. Plant Science, 8: 1429.
Yuan, L. and Li, R. (2020). Metabolic engineering a model oilseed camelina sativa for the sustainable production of high-value designed oils. Frontiers in Plant Science, 11: 11. https://doi.org/10.3389/fpls.2020.00011
Zuo, J., Niu, Q.W., Frugis, G. and Chua, N.H. (2002). The WUSCHEL gene promotes vegetative-to-embryonic transition in Arabidopsis. Plant Journal, 30: 349–359. https://doi.org/10.1046/j.1365-313X.2002.01289.x